Showing papers by "Chul Ho Kim published in 2014"
TL;DR: For consolidated bioprocessing (CBP), components of Jerusalem artichoke ( Helianthus tuberosus L.) tubers and stalks as a potential bioenergy crop were analyzed as carbon and nutrient sources, respectively as mentioned in this paper.
45 citations
TL;DR: To increase the yield of 2-butanol, the 2,3-butanediol pathway from the recombinant strain is eliminated by inactivating α-acetolactate decarboxylase (adc) and the yield is improved from 160 to 320 mg l−1.
Abstract: Klebsiella pneumoniae was engineered to produce 2-butanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (ilvIH), keto-acid reducto-isomerase (ilvC) and dihydroxy-acid dehydratase (ilvD) from K. pneumoniae, and α-ketoisovalerate decarboxylase (kivd) and alcohol dehydrogenase (adhA) from Lactococcus lactis. Engineered K. pneumonia, ∆ldhA/pBR-iBO (ilvIH–ilvC–ilvD–kivd–adhA), produced 2-butanol (160 mg l−1) from crude glycerol. To increase the yield of 2-butanol, we eliminated the 2,3-butanediol pathway from the recombinant strain by inactivating α-acetolactate decarboxylase (adc). This further engineering step improved the yield of 2-butanol from 160 to 320 mg l−1. This represents the first successful attempt to produce 2-butanol from crude glycerol.
17 citations
TL;DR: To increase the yield of isobutanol, the 2,3-butanediol pathway from the recombinant strain was eliminated by inactivating α-acetolactate decarboxylase (adc) and the yield was improved from 160 to 320 mg l−1.
Abstract: Klebsiella pneumoniae was engineered to produce isobutanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (ilvIH), keto-acid reducto-isomerase (ilvC) and dihydroxy-acid dehydratase (ilvD) from K. pneumoniae, and α-ketoisovalerate decarboxylase (kivd) and alcohol dehydrogenase (adhA) from Lactococcus lactis. Engineered K. pneumonia, ∆ldhA/pBR-iBO (ilvIH–ilvC–ilvD–kivd–adhA), produced isobutanol (160 mg l−1) from crude glycerol. To increase the yield of isobutanol, we eliminated the 2,3-butanediol pathway from the recombinant strain by inactivating α-acetolactate decarboxylase (adc). This further engineering step improved the yield of isobutanol from 160 to 320 mg l−1. This represents the first successful attempt to produce isobutanol from crude glycerol.
9 citations
TL;DR: This is the first report of the production of mixed acids from the red algae G. amansii, and enrichment cultures of anaerobic digester sludge and tidal field soil compared with fresh sludge increased mixed acids production level 1.6-fold.
6 citations
Patent•
28 Apr 2014
TL;DR: In this paper, a monascus strain suitable for liquid fermentation of an ultra-fine pulverized substrate and monascus liquid fermentation process, and more specifically, to Monascus sp. RY1 strain, is presented.
Abstract: The present invention relates to a monascus strain suitable for liquid fermentation of an ultra-fine pulverized substrate and a monascus liquid fermentation process, and more specifically, to Monascus sp. RY1 strain suitable for liquid fermentation of a substrate automized by using an ultra-fine grinding technique and a monascus liquid fermentation process using the same. A liquid fermentation process according to the present invention can easily ferment monascus, reduce fermentation time, and prevent pollution thereby being variously used in alcoholic fermentation, acetic acid fermentation, lactic acid fermentation, fermented soybean fermentation, etc. and accordingly being applicable for well-being food development using monascus.
1 citations
Patent•
17 Apr 2014
TL;DR: In this paper, a raw rice wine manufacturing method using dioscorea batatas-monascus and more specifically, a raw Rice wine making method which primary ferments with monascus and secondary ferments by using yeast fungus.
Abstract: The present invention relates to a raw rice wine manufacturing method using dioscorea batatas-monascus and, more specifically, to a raw rice wine manufacturing method which primary ferments with monascus using dioscorea batatas as a main ingredient and secondary ferments by using yeast fungus, and dioscorea batatas-monascus raw rice wine manufactured by the method. Dioscorea batatas-monascus raw rice wine according to the present invention has excellent antioxidant effects and contains a high content of dietary fibers and proteins thereby having good nutrition and efficacy compared to general raw rice wine. In addition, a delight flavor of dioscorea batatas-monascus is added thereby more improving flavors of the raw rice wine.
1 citations