Chunfu Xu

TL;DR: The computational design of a 25-nanometre icosahedral nanocage that self-assembles from trimeric protein building blocks is described, and such robust and customizable nanocages should have considerable utility in targeted drug delivery, vaccine design and synthetic biology.

TL;DR: A general approach for refining protein structure models on the basis of cryo-electron microscopy maps with near-atomic resolution that integrates Monte Carlo sampling with local density-guided optimization, Rosetta all-atom refinement and real-space B-factor fitting is described.

TL;DR: The ability to design extensive hydrogen-bond networks with atomic accuracy enables the programming of protein interaction specificity for a broad range of synthetic biology applications; more generally, the results demonstrate that, even with the tremendous diversity observed in nature, there are fundamentally new modes of interaction to be discovered in proteins.

TL;DR: Protein design expands the repertoire of coiled-coil structures to α-helical barrels and hyperstable helical bundles, and describes a procedure for designing proteins with backbones produced by varying the parameters in the Crick coiled coil–generating equations.

TL;DR: This investigation provides a design rubric for fabrication of structurally defined, peptide-based nanosheets using the principles of solution-based self-assembly facilitated through complementary electrostatic interactions.