C
Chunlai Chen
Researcher at Tsinghua University
Publications - 60
Citations - 1563
Chunlai Chen is an academic researcher from Tsinghua University. The author has contributed to research in topics: Förster resonance energy transfer & Transfer RNA. The author has an hindex of 20, co-authored 50 publications receiving 1290 citations. Previous affiliations of Chunlai Chen include University of Pennsylvania & Peking University.
Papers
More filters
Journal ArticleDOI
Aptamer biosensor for protein detection using gold nanoparticles
TL;DR: Under experimental conditions, the recognition of protein by the complex of dye-labeled DNA hybridized with aptamer that is immobilized on GNPs shows the best character in protein detection.
Journal ArticleDOI
Single-molecule fluorescence measurements of ribosomal translocation dynamics.
Chunlai Chen,Benjamin Stevens,Jaskarin Kaur,Diana Cabral,Hanqing Liu,Yuhong Wang,Yuhong Wang,Haibo Zhang,Gabriel Rosenblum,Zeev Smilansky,Yale E. Goldman,Barry S. Cooperman +11 more
TL;DR: Single-molecule fluorescence resonance energy transfer (smFRET) is employed to study structural dynamics over the first two elongation cycles of protein synthesis, using ribosomes containing either Cy3-labeled ribosomal protein L11 and A- or P-site Cy5- labeled tRNAs.
Journal ArticleDOI
Dynamics of translation by single ribosomes through mRNA secondary structures
Chunlai Chen,Haibo Zhang,Steven L. Broitman,Michael Reiche,Ian Farrell,Barry S. Cooperman,Yale E. Goldman +6 more
TL;DR: Single-molecule fluorescence resonance energy transfer is used to determine reaction rates for specific steps within the elongation cycle as the Escherichia coli ribosome encounters stem-loop or pseudoknot mRNA secondary structures, finding that unfolding of mRNAsecondary structures is more closely coupled to E-site tRNA dissociation than to tRNA translocation.
Journal ArticleDOI
Influence of secondary structure on kinetics and reaction mechanism of DNA hybridization
TL;DR: This work investigates the kinetics of DNA hybridization/denaturation via fluorescence resonance energy transfer (FRET) on perfectly matched and single-base-mismatched DNA strands and proposes a reaction mechanism about how the melting of secondary structure influences the hybridization process.
Journal ArticleDOI
Kinetics and thermodynamics of DNA hybridization on gold nanoparticles
TL;DR: It is found that the secondary structure of a DNA hairpin inhibits the interaction between GNPs and DNA and enhances the stability of the DNA hairpins adhered to GNPs.