C
Cyril Barinka
Researcher at Academy of Sciences of the Czech Republic
Publications - 70
Citations - 2507
Cyril Barinka is an academic researcher from Academy of Sciences of the Czech Republic. The author has contributed to research in topics: Glutamate carboxypeptidase II & Medicine. The author has an hindex of 25, co-authored 54 publications receiving 1984 citations. Previous affiliations of Cyril Barinka include University of Illinois at Chicago & National Institutes of Health.
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Journal ArticleDOI
Structure of glutamate carboxypeptidase II, a drug target in neuronal damage and prostate cancer.
Jeroen R. Mesters,Cyril Barinka,Weixing Li,Takashi Tsukamoto,Pavel Majer,Barbara S. Slusher,Jan Konvalinka,Rolf Hilgenfeld +7 more
TL;DR: Three‐dimensional structures presented here reveal an induced‐fit substrate‐binding mode of this key enzyme and provide essential information for the design of GCPII inhibitors useful in the treatment of neuronal diseases and prostate cancer.
Journal ArticleDOI
Interactions between Human Glutamate Carboxypeptidase II and Urea-Based Inhibitors: Structural Characterization†
Cyril Barinka,Youngjoo Byun,Crystal L. Dusich,Sangeeta Ray Banerjee,Ying Chen,Mark Castanares,Alan P. Kozikowski,Ronnie C. Mease,Martin G. Pomper,Jacek Lubkowski +9 more
TL;DR: X-ray structures of four complexes between human GCPII and urea-based inhibitors at high resolution are determined and a hydrophobic pocket accessory to the S1 site is identified that can be exploited for structure-based design of novel G CPII inhibitors with increased lipophilicity.
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A Remote Arene-Binding Site on Prostate Specific Membrane Antigen Revealed by Antibody-Recruiting Small Molecules.
Andrew Zhang,Ryan P. Murelli,Cyril Barinka,Julien Michel,Alexandra Cocleaza,William L. Jorgensen,Jacek Lubkowski,David Spiegel +7 more
TL;DR: In-depth biochemical, crystallographic, and computational investigations reveal a previously unreported arene-binding site on PSMA, which is believed to participate in an aromatic stacking interaction with ARMs, which provides critical insights into the design of PSMA-targeted small molecules.
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Substrate specificity, inhibition and enzymological analysis of recombinant human glutamate carboxypeptidase II.
Cyril Barinka,Markéta Rinnová,Pavel Šácha,Camilo Rojas,Pavel Majer,Barbara S. Slusher,Jan Konvalinka +6 more
TL;DR: In this paper, an extracellular portion of human glutamate carboxypeptidase II (amino acids 44-750) was obtained from Drosophila Schneider's cells and its purification to homogeneity was established.
Journal ArticleDOI
Expression of glutamate carboxypeptidase II in human brain.
Pavel Šácha,Josef Zamecnik,Cyril Barinka,Klára Hlouchová,Klára Hlouchová,Ales Vicha,Petra Mlčochová,Petra Mlčochová,Ivan Hilgert,Tomas Eckschlager,Jan Konvalinka,Jan Konvalinka +11 more
TL;DR: Investigation of the activity and expression of GCPII in various compartments of the human brain using a radiolabeled substrate of the enzyme and the novel monoclonal antibody GCP-04 shows that this antibody is more sensitive in immunoblots than the widely used antibody 7E11.