Dana R Kadavy

TL;DR: Evidence of the utility of amplicon sequencing is offered by demonstrating that the current versions of MinION™ technology can accurately identify and differentiate both viral and bacterial species present within biological samples via Amplicon sequencing.

TL;DR: Criteria that should be considered for HTS validation for microbial forensics are presented here and comprise a foundation for investigators to establish, validate and implement HTS as a tool in microbialForensics, enhancing public safety and national security.

TL;DR: A proof-of-concept was demonstrated by integrating both platforms in a hybrid assembly that leverages solely mixture data to accurately reconstruct full mitochondrial genomes.

TL;DR: A novel sample-to-answer workflow was assembled to enable rapid clinical detection of causative pathogens of bacteremia in whole blood utilizing metagenomic sequence data captured by the MinION, and may permit physicians to better target antibiotic therapies without turning to broad spectrum antibiotics, which may further propagate resistant strains.

TL;DR: An analytical method specifically designed to rapidly detect a set of target organisms in mixed samples that achieves a high degree of species- and strain-specificity by aligning short sequence reads to the genomes of near neighbor organisms, as well as that of the target.