J
Joseph T. Clark
Researcher at Stanford University
Publications - 7
Citations - 1074
Joseph T. Clark is an academic researcher from Stanford University. The author has contributed to research in topics: Genome editing & CRISPR. The author has an hindex of 4, co-authored 7 publications receiving 853 citations.
Papers
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Journal ArticleDOI
Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells
Ayal Hendel,Rasmus O. Bak,Joseph T. Clark,Andrew Kennedy,Daniel E. Ryan,Subhadeep Roy,Israel Steinfeld,Benjamin D. Lunstad,Robert J. Kaiser,Alec B. Wilkens,Rosa Bacchetta,Anya Tsalenko,Douglas J. Dellinger,Laurakay Bruhn,Matthew H. Porteus +14 more
TL;DR: Co-delivering chemically modified sgRNAs with Cas9 mRNA or protein is an efficient RNA- or ribonucleoprotein (RNP)-based delivery method for the CRISPR-Cas system, without the toxicity associated with DNA delivery.
Journal ArticleDOI
Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing.
Ayal Hendel,Eric J. Kildebeck,Eli J. Fine,Joseph T. Clark,Niraj Punjya,Vittorio Sebastiano,Gang Bao,Matthew H. Porteus +7 more
TL;DR: This work has developed a method for quantifying individual genome-editing outcomes at any site of interest with single-molecule real-time (SMRT) DNA sequencing and shows that this approach can be applied at various loci using multiple engineered nuclease platforms.
Patent
Chemically modified guide rnas for crispr/cas-mediated gene regulation
Matthew H. Porteus,Ayal Hendel,Joseph T. Clark,Rasmus O. Bak,Daniel E. Ryan,Douglas J. Dellinger,Robert J. Kaiser,Joel Myerson +7 more
TL;DR: In this paper, the authors proposed methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (i.e., target DNA or target RNA) in a cell.
Journal ArticleDOI
CRISPR/Cas9 Genome Engineering in Engraftable Human Brain-Derived Neural Stem Cells.
Daniel P. Dever,Samantha G. Scharenberg,Joab Camarena,Eric J. Kildebeck,Joseph T. Clark,Renata M. Martin,Rasmus O. Bak,Yuming Tang,Monika Dohse,Johannes Birgmeier,Karthik A. Jagadeesh,Gill Bejerano,Ann Tsukamoto,Natalia Gomez-Ospina,Nobuko Uchida,Matthew H. Porteus +15 more
TL;DR: These GE-NSCs have the potential to be an investigational cell and gene therapy for a range of neurodegenerative disorders and injuries of the central nervous system, including lysosomal storage disorders.
Patent
Genome editing of human neural stem cells using nucleases
Joseph T. Clark,Daniel P. Dever,Eric J. Kildebeck,Matthew H. Porteus,Ann Tsukamoto,Nobuko Uchida +5 more
TL;DR: In this article, the authors proposed methods for generating a genetically modified human neural stem cell, genetically modified HNS cells, and pharmaceutical compositions comprising the genetically modified Human Neural Stem cells.