M
Matthew H. Porteus
Researcher at Stanford University
Publications - 222
Citations - 20802
Matthew H. Porteus is an academic researcher from Stanford University. The author has contributed to research in topics: Genome editing & Stem cell. The author has an hindex of 61, co-authored 201 publications receiving 17501 citations. Previous affiliations of Matthew H. Porteus include Cardiovascular Institute of the South & University of Texas System.
Papers
More filters
Journal ArticleDOI
Highly efficient endogenous human gene correction using designed zinc-finger nucleases
Fyodor D. Urnov,Jeffrey C. Miller,Ya-Li Lee,Christian Beauséjour,Jeremy M. Rock,Sheldon Augustus,Andrew C. Jamieson,Matthew H. Porteus,Philip D. Gregory,Michael C. Holmes +9 more
TL;DR: It is shown that zinc-finger nucleases designed against an X-linked severe combined immune deficiency mutation in the IL2Rγ gene yielded more than 18% gene-modified human cells without selection, raising the possibility of strategies based on zinc- finger nucleases for the treatment of disease.
Journal ArticleDOI
Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells
Ayal Hendel,Rasmus O. Bak,Joseph T. Clark,Andrew Kennedy,Daniel E. Ryan,Subhadeep Roy,Israel Steinfeld,Benjamin D. Lunstad,Robert J. Kaiser,Alec B. Wilkens,Rosa Bacchetta,Anya Tsalenko,Douglas J. Dellinger,Laurakay Bruhn,Matthew H. Porteus +14 more
TL;DR: Co-delivering chemically modified sgRNAs with Cas9 mRNA or protein is an efficient RNA- or ribonucleoprotein (RNP)-based delivery method for the CRISPR-Cas system, without the toxicity associated with DNA delivery.
Journal ArticleDOI
Chimeric Nucleases Stimulate Gene Targeting in Human Cells
TL;DR: Homodimers of CNs are potent stimulators of gene targeting in human somatic cells and can stimulate homologous recombination by over 1000-fold.
Journal ArticleDOI
Activation of proto-oncogenes by disruption of chromosome neighborhoods
Denes Hnisz,Abraham S. Weintraub,Daniel S. Day,Anne-Laure Valton,Rasmus O. Bak,Charles H. Li,Johanna Goldmann,Bryan R. Lajoie,Zi Peng Fan,Alla A. Sigova,Jessica Reddy,Diego Borges-Rivera,Tong Ihn Lee,Rudolf Jaenisch,Matthew H. Porteus,Job Dekker,Job Dekker,Richard A. Young +17 more
TL;DR: Insulated neighborhoods in T cell acute lymphoblastic leukemia (T-ALL) are mapped and it is found that tumor cell genomes contain recurrent microdeletions that eliminate the boundary sites of insulated neighborhoods containing prominent T-ALL proto-oncogenes.
Journal ArticleDOI
Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification.
Morgan L. Maeder,Stacey Thibodeau-Beganny,Anna Osiak,David A. Wright,Reshma M. Anthony,Magdalena Eichtinger,Tao Jiang,Jonathan E. Foley,Ronnie J. Winfrey,Jeffrey A. Townsend,Erica Unger-Wallace,Jeffry D. Sander,Felix Müller-Lerch,Fengli Fu,Joseph Pearlberg,Carl Göbel,Justin P. Dassie,Shondra M. Pruett-Miller,Matthew H. Porteus,Dennis C. Sgroi,A. John Iafrate,Drena Dobbs,Paul B. McCray,Toni Cathomen,Daniel F. Voytas,J. Keith Joung +25 more
TL;DR: OP (Oligomerized Pool ENgineering), a rapid, publicly available strategy for constructing multifinger arrays, which is more effective than the previously published modular assembly method and provides an "open-source" method for rapidly engineering highly active zinc-finger arrays.