D
David A. Kulesh
Researcher at United States Army Medical Research Institute of Infectious Diseases
Publications - 17
Citations - 1259
David A. Kulesh is an academic researcher from United States Army Medical Research Institute of Infectious Diseases. The author has contributed to research in topics: TaqMan & Variola virus. The author has an hindex of 13, co-authored 17 publications receiving 1114 citations.
Papers
More filters
Journal ArticleDOI
Immunogenicity of a highly attenuated MVA smallpox vaccine and protection against monkeypox
Patricia L. Earl,Jeffrey L. Americo,Linda S. Wyatt,Leigh Anne Eller,J. Charles Whitbeck,Gary H. Cohen,Roselyn J. Eisenberg,Chris Hartmann,David L. Jackson,David A. Kulesh,Mark J. Martinez,David M. Miller,Eric M. Mucker,Joshua D. Shamblin,Susan H. Zwiers,John W. Huggins,Peter B. Jahrling,Bernard Moss +17 more
TL;DR: The highly attenuated modified vaccinia virus Ankara (MVA) with the licensed Dryvax vaccine in a monkey model is compared and unimmunized animals developed more than 500 pustular skin lesions and became gravely ill or died, whereas vaccinated animals were healthy and asymptomatic.
Journal ArticleDOI
Comprehensive Panel of Real-Time TaqMan™ Polymerase Chain Reaction Assays for Detection and Absolute Quantification of Filoviruses, Arenaviruses, and New World Hantaviruses
Adrienne R. Trombley,Leslie Wachter,Jeffrey Garrison,Valerie A. Buckley-Beason,Jordan B. Jahrling,Lisa E. Hensley,Randal J. Schoepp,David A. Norwood,Augustine Goba,Joseph N. Fair,David A. Kulesh +10 more
TL;DR: These real-time hemorrhagic fever virus assays are qualitative (presence of target), they are also quantitative (measure a single DNA/RNA target sequence in an unknown sample and express the final results as an absolute value) and can be used in viral load, vaccine, and antiviral drug studies.
Journal ArticleDOI
Smallpox and pan-Orthopox Virus Detection by Real-Time 3′-Minor Groove Binder TaqMan Assays on the Roche LightCycler and the Cepheid Smart Cycler Platforms
David A. Kulesh,Robert O. Baker,Bonnie M. Loveless,David A. Norwood,Susan H. Zwiers,Eric M. Mucker,Chris Hartmann,Rafael Herrera,David M. Miller,Deanna R. Christensen,Leonard P. Wasieloski,John W. Huggins,Peter B. Jahrling +12 more
TL;DR: These real-time PCR assays represent a battery of tests to screen for and confirm the presence of variola virus DNA and had nearly 100% sensitivity on both machines with samples above the LOD (>12 gene copies).
Journal ArticleDOI
Cynomolgus Macaque as an Animal Model for Severe Acute Respiratory Syndrome
James V. Lawler,Timothy P. Endy,Lisa E. Hensley,Aura R. Garrison,Elizabeth A. Fritz,May Lesar,Ralph S. Baric,David A. Kulesh,David A. Norwood,Leonard P. Wasieloski,Melanie P. Ulrich,Tom Slezak,Elizabeth A. Vitalis,John W. Huggins,Peter B. Jahrling,Jason Paragas +15 more
TL;DR: SARS-CoV infection of cynomolgus macaques did not reproduce the severe illness seen in the majority of adult human cases of Sars; however, the results suggest similarities to the milder syndrome of SARS- CoV infection characteristically seen in young children.
Journal ArticleDOI
Detection of Biological Threat Agents by Real-Time PCR: Comparison of Assay Performance on the R.A.P.I.D., the LightCycler, and the Smart Cycler Platforms
Deanna R. Christensen,Laurie J. Hartman,Bonnie M. Loveless,Melissa S. Frye,Michelle A. Shipley,Deanna L. Bridge,Michelle J. Richards,Rebecca Susan Kaplan,Jeffrey Garrison,Carson Baldwin,David A. Kulesh,David A. Norwood +11 more
TL;DR: Assays for DNA templates from biological threat agents demonstrated similar performance, sensitivity, and specificity on all 3 platforms.