D
David K. Liscombe
Researcher at University of Calgary
Publications - 25
Citations - 1644
David K. Liscombe is an academic researcher from University of Calgary. The author has contributed to research in topics: Benzylisoquinoline & Catharanthus roseus. The author has an hindex of 16, co-authored 22 publications receiving 1439 citations. Previous affiliations of David K. Liscombe include Salk Institute for Biological Studies & Massachusetts Institute of Technology.
Papers
More filters
Journal ArticleDOI
Molecular cloning and characterization of norcoclaurine synthase, an enzyme catalyzing the first committed step in benzylisoquinoline alkaloid biosynthesis
TL;DR: The novel catalytic functions of NCS and HYP1 define a new class of plant secondary metabolic enzymes within the Bet v 1 and PR10 protein families.
Journal ArticleDOI
Architectures, mechanisms and molecular evolution of natural product methyltransferases
TL;DR: It is shown how a widespread protein fold evolved to accommodate chemically diverse methyl acceptors and to catalyse disparate mechanisms suited to the physiochemical properties of the target substrates, suggesting that NPMTs may serve as starting points for generating new biocatalysts.
Journal ArticleDOI
Evidence for the monophyletic evolution of benzylisoquinoline alkaloid biosynthesis in angiosperms.
TL;DR: Phylogenetic analyses of NCS, berberine bridge enzyme and several O-methyltransferases suggest a latent molecular fingerprint for BIA biosynthesis in angiosperms not known to accumulate such alkaloids, and support the monophyletic origin of Bia biosynthesis prior to the emergence of the eudicots
Journal ArticleDOI
Development of transcriptomic resources for interrogating the biosynthesis of monoterpene indole alkaloids in medicinal plant species.
Elsa Góngora-Castillo,Kevin L. Childs,Greg Fedewa,John P. Hamilton,David K. Liscombe,Maria Magallanes-Lundback,Kranthi K. Mandadi,Ezekiel Nims,Weerawat Runguphan,Brieanne Vaillancourt,Marina Varbanova-Herde,Dean DellaPenna,Thomas D. McKnight,Sarah E. O'Connor,Sarah E. O'Connor,C. Robin Buell +15 more
TL;DR: Large-scale transcriptome sequence and expression profiles for three species of Asterids that produce medicinally important MIAs are generated, providing a rich resource for understanding plant specialized metabolism, and promotes realization of innovative production systems for plant-derived pharmaceuticals.
Journal ArticleDOI
A Stereoselective Hydroxylation Step of Alkaloid Biosynthesis by a Unique Cytochrome P450 in Catharanthus roseus
Lesley-Ann Giddings,David K. Liscombe,John P. Hamilton,Kevin L. Childs,Dean DellaPenna,C. Robin Buell,Sarah E. O'Connor +6 more
TL;DR: The discovery of CYP71BJ1, which forms part of the pathway leading to 19-O-acetylhörhammericine, will help illuminate how this branch point in alkaloid biosynthesis is controlled in C. roseus.