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Deepak Reyon
Researcher at Harvard University
Publications - 53
Citations - 17227
Deepak Reyon is an academic researcher from Harvard University. The author has contributed to research in topics: Genome editing & Transcription activator-like effector nuclease. The author has an hindex of 33, co-authored 51 publications receiving 15306 citations. Previous affiliations of Deepak Reyon include Iowa State University & University of Minnesota.
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Journal ArticleDOI
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells.
Yanfang Fu,Jennifer A Foden,Cyd Khayter,Morgan L. Maeder,Deepak Reyon,J. Keith Joung,Jeffry D. Sander +6 more
TL;DR: It is found that single and double mismatches are tolerated to varying degrees depending on their position along the guide RNA (gRNA)-DNA interface, and off-target cleavage of CRISPR-associated (Cas)9-based RGNs is characterized.
Journal ArticleDOI
Efficient genome editing in zebrafish using a CRISPR-Cas system
Woong Y. Hwang,Yanfang Fu,Deepak Reyon,Morgan L. Maeder,Shengdar Q. Tsai,Jeffry D. Sander,Randall T. Peterson,Randall T. Peterson,Jing-Ruey J. Yeh,J. Keith Joung +9 more
TL;DR: It is shown that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.
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Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
TL;DR: It is reported that truncated gRNAs, with shorter regions of target complementarity <20 nucleotides in length, can decrease undesired mutagenesis at some off-target sites by 5,000-fold or more without sacrificing on-target genome editing efficiencies.
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FLASH assembly of TALENs for high-throughput genome editing
TL;DR: The fast ligation-based automatable solid-phase high-throughput (FLASH) system is described, a rapid and cost-effective method for large-scale assembly of TALENs and it is demonstrated that FLASH facilitates high- throughput genome editing at a scale not currently possible with other genome modification technologies.
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Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing
Shengdar Q. Tsai,Nicolas Wyvekens,Cyd Khayter,Jennifer A Foden,Vishal Thapar,Deepak Reyon,Mathew J. Goodwin,Martin J. Aryee,J. Keith Joung +8 more
TL;DR: D dimeric RNA-guided FokI nucleases (RFNs) are described that can recognize extended sequences and edit endogenous genes with high efficiencies in human cells and are likely to be useful in applications that require highly precise genome editing.