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Deepak Reyon
Researcher at Harvard University
Publications - 53
Citations - 17227
Deepak Reyon is an academic researcher from Harvard University. The author has contributed to research in topics: Genome editing & Transcription activator-like effector nuclease. The author has an hindex of 33, co-authored 51 publications receiving 15306 citations. Previous affiliations of Deepak Reyon include Iowa State University & University of Minnesota.
Papers
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Journal ArticleDOI
Predicting success of oligomerized pool engineering (OPEN) for zinc finger target site sequences
Jeffry D. Sander,Jeffry D. Sander,Deepak Reyon,Morgan L. Maeder,Jonathan E. Foley,Stacey Thibodeau-Beganny,Xiaohong Li,Maureen R. Regan,Elizabeth J. Dahlborg,Matthew J Goodwin,Fengli Fu,Daniel F. Voytas,J. Keith Joung,Drena Dobbs +13 more
TL;DR: ZiFOpT, a machine learning classifier trained to identify DNA sequences amenable for targeting by OPEN-generated zinc finger arrays, can guide users to target sites that are most likely to function successfully in vivo, substantially reducing the experimental effort required.
Journal ArticleDOI
Response to "Unexpected mutations after CRISPR-Cas9 editing in vivo".
Christine D. Wilson,Timothy Fennell,Anne Bothmer,Morgan L. Maeder,Deepak Reyon,Cecilia Cotta-Ramusino,Cecilia Fernández,Eugenio Marco,Luis A. Barrera,Hariharan Jayaram,Charles F Albright,Gerald F. Cox,George M. Church,Vic E. Myer +13 more
Journal ArticleDOI
IκB Kinase β (IKBKB) Mutations in Lymphomas That Constitutively Activate Canonical Nuclear Factor κB (NFκB) Signaling
Xin Kai,Vasant Chellappa,Carlos Donado,Deepak Reyon,Yurie Sekigami,Dalya Ataca,Abner Louissaint,Hamid Mattoo,J. Keith Joung,Shiv Pillai +9 more
TL;DR: It is shown here that K171E IKK β and K171T IKKβ represent kinases that are constitutively active even in the absence of activation loop phosphorylation, which has broad implications for the function of positively charged residues in all activation loop-dependent kinases.
Book ChapterDOI
Targeted Mutagenesis in Zebrafish Using CRISPR RNA-Guided Nucleases.
Woong Y. Hwang,Yanfang Fu,Deepak Reyon,Andrew P. W. Gonzales,J. Keith Joung,Jing-Ruey J. Yeh +5 more
TL;DR: In this article, the authors describe protocols for creating insertion or deletion (indel) mutations or precise sequence modifications in zebrafish genes using customizable CRISPR-Cas9 RNA-guided nucleases (RGNs).
Journal ArticleDOI
Genome editing of factor X in zebrafish reveals unexpected tolerance of severe defects in the common pathway
Zhilian Hu,Yang Liu,Michael C. Huarng,Marzia Menegatti,Marzia Menegatti,Deepak Reyon,Megan S. Rost,Zachary G. Norris,Catherine E. Richter,Alexandra N. Stapleton,Neil C. Chi,Flora Peyvandi,J. Keith Joung,Jordan A. Shavit +13 more
TL;DR: It is shown that ablation of zebrafish f10 by using genome editing with transcription activator-like effector nucleases results in a major embryonic hemostatic defect, however, widespread hemorrhage and subsequent lethality does not occur until later stages, with absence of any detectable defect in vascular development.