D
Dennis Shields
Researcher at Albert Einstein College of Medicine
Publications - 59
Citations - 2648
Dennis Shields is an academic researcher from Albert Einstein College of Medicine. The author has contributed to research in topics: Golgi apparatus & Secretory pathway. The author has an hindex of 30, co-authored 59 publications receiving 2571 citations.
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Journal ArticleDOI
Phospholipase D Stimulates Release of Nascent Secretory Vesicles from the trans-Golgi Network
Ye-Guang Chen,Anirban Siddhanta,Cary D. Austin,Scott M. Hammond,Tsung Chang Sung,Michael A. Frohman,Andrew J. Morris,Dennis Shields +7 more
TL;DR: It is demonstrated that immunoaffinity-purified human PLD1 stimulated nascent secretory vesicle budding from the TGN and ARF-1 stimulated endogenous PLD activity in Golgi membranes approximately threefold and this activation correlated with its enhancement of vesicles budding.
Journal ArticleDOI
Intracellular Localization of Phospholipase D1 in Mammalian Cells
Zachary Freyberg,David A. Sweeney,Anirban Siddhanta,Sylvain G. Bourgoin,Michael A. Frohman,Dennis Shields +5 more
TL;DR: The data show that the intracellular localization of PLD1 is consistent with a role in vesicle trafficking from the Golgi apparatus and suggest that it also functions in the cell nucleus.
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A caspase cleavage fragment of p115 induces fragmentation of the Golgi apparatus and apoptosis
TL;DR: In vivo expression of the COOH-terminal fragment in the presence of caspase inhibitors, or upon coexpression with a cleavage-resistant mutant of p115, showed that p115 degradation plays a key role in amplifying the apoptotic response independently of Golgi fragmentation.
Journal ArticleDOI
The propeptide of preprosomatostatin mediates intracellular transport and secretion of alpha-globin from mammalian cells.
T J Stoller,Dennis Shields +1 more
TL;DR: It is concluded that the somatostatin propeptide mediated transport of alpha-globin from the endoplasmic reticulum to the trans-Golgi network by protecting molecules from degradation and in addition, facilitated packaging ofalpha- globin into vesicles whose secretion was stimulated by cAMP.
Journal ArticleDOI
Secretory Vesicle Budding from the Trans-Golgi Network Is Mediated by Phosphatidic Acid Levels *
Anirban Siddhanta,Dennis Shields +1 more
TL;DR: The data demonstrate that in mammalian cells accumulation of PA rather than DAG is a key step in regulating budding of secretory vesicles from the trans-Golgi network.