Diana M. Harris

TL;DR: Genes predicted to encode transporters were strongly overrepresented among the genes transcriptionally upregulated under conditions that stimulate penicillinG production, illustrating potential for future genomics-driven metabolic engineering.

TL;DR: This study demonstrates the usefulness of combinatorial transcriptome analysis in chemostat cultures to dissect effects of biological and process parameters on gene expression regulation and provides for the first time clear-cut target genes for metabolic engineering, beyond the three genes of the β-lactam pathway.

TL;DR: In this paper, a strain of penicillium chrysogenum was successfully engineered to produce a novel carbamoylated cephalosporin that can be used as a synthon for semi-synthetic cephanosporins, and a combinatorial chemostat-based transcriptome study was conducted to identify genes for enzymes involved in mitochondrial and peroxisomal beta-oxidation pathways.

TL;DR: In this study, NADPH metabolism was investigated in glucose-limited chemostat cultures of an industrial P. chrysogenum strain and isolated mitochondria showed high rates of antimycin A-sensitive respiration of NADPH, thus indicating the presence of a mitochondrial NADPH dehydrogenase that oxidises cytosolic NADPH.

TL;DR: Results demonstrate that, in principle, mixed-substrate feeding can be used to increase the yield on a carbon source of assimilatory products such as β-lactams by cofeeding of an auxiliary substrate that acts as an energy source but not as a carbon substrate.