D
Diana V. Pastrana
Researcher at National Institutes of Health
Publications - 57
Citations - 4625
Diana V. Pastrana is an academic researcher from National Institutes of Health. The author has contributed to research in topics: Merkel cell polyomavirus & Antibody. The author has an hindex of 30, co-authored 56 publications receiving 4170 citations. Previous affiliations of Diana V. Pastrana include Government of the United States of America & Laboratory of Molecular Biology.
Papers
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Journal ArticleDOI
Merkel Cell Polyomavirus and Two Previously Unknown Polyomaviruses Are Chronically Shed from Human Skin
Rachel M. Schowalter,Diana V. Pastrana,Katherine A. Pumphrey,Adam L. Moyer,Christopher B. Buck +4 more
TL;DR: An improved rolling circle amplification (RCA) technique to isolate circular DNA viral genomes from human skin swabs identified two previously unknown polyomavirus species that are named human polyomvirus-6 (HPyV6) and HPyV7 and indicate that infection or coinfection with these three skin-tropicpolyomaviruses is very common.
Journal ArticleDOI
Efficient Intracellular Assembly of Papillomaviral Vectors
TL;DR: The results suggest that the intracellular assembly of papillomavirus structural proteins around heterologous reporter plasmids is surprisingly promiscuous and may be driven primarily by a size discrimination mechanism.
Journal ArticleDOI
Reactivity of human sera in a sensitive, high-throughput pseudovirus-based papillomavirus neutralization assay for HPV16 and HPV18
Diana V. Pastrana,Christopher B. Buck,Yuk Ying S. Pang,Cynthia D. Thompson,Philip E. Castle,Peter C. FitzGerald,Susanne K. Kjaer,Douglas R. Lowy,John T. Schiller +8 more
TL;DR: The SEAP pseudovirus-based neutralization assay should be a practical method for quantifying potentially protective antibody responses in HPV natural history and prophylactic vaccine studies.
Journal ArticleDOI
Human Merkel cell polyomavirus infection II. MCV is a common human infection that can be detected by conformational capsid epitope immunoassays
Yanis L. Tolstov,Diana V. Pastrana,Huichen Feng,J. C. Becker,Frank J. Jenkins,Stergios J. Moschos,Yuan Chang,Christopher B. Buck,Patrick S. Moore +8 more
TL;DR: It is demonstrated that Merkel cell polyomavirus is a widespread but previously unrecognized human infection and MCC patients have a markedly elevated MCV IgG response compared with control patients.
Book ChapterDOI
Generation of HPV pseudovirions using transfection and their use in neutralization assays.
TL;DR: This chapter outlines a simple method for production of PsV and their use in a high-throughput papillomavirus neutralization assay, which has similar analytic sensitivity to, and higher specificity than, a standard VLP-based enzyme-linked immunosorbent assay (ELISA).