Dominika Malinska

TL;DR: The use of chemical probes for the rapid detection of ROS in intact and permeabilized adherent cells by fluorescence microscopy and fluorometry is described and methods to assess the production of specific ROS in various fields of investigation, including the study of oncometabolism are recommended.

TL;DR: Melatonin attenuated diabetes‐induced alterations in glutathione redox state and HFR levels, normalized creatinine concentration and diminished urea content in serum and the examined organs, and the indole‐induced increase in the activities of the enzymes of glutathion metabolism might be of importance for antioxidative action of melatonin under diabetic conditions.

TL;DR: The present review summarizes the current knowledge of basic MAM biology, composition and action, and discusses the potential connections supporting the idea that MAM are significant players in longevity.

TL;DR: Two strategies to measure ROS are described: one approach to assess general oxidant levels using the chemical reporter CM-H2DCFDA (5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate), and a second approach allowing more specific analysis of cytosolic hydrogen peroxide (H2O2) levels using protein-based sensors (HyPer and SypHer).

TL;DR: This critical review summarizes literature findings about the mitochondrial production of ROS, the action of KCO's on mitochondrial ROS production and the putative link to the cytoprotective action of these drugs.