Mariusz R. Wieckowski

TL;DR: It is demonstrated that VDAC1 is physically linked to the endoplasmic reticulum Ca2+-release channel inositol 1,4,5-trisphosphate receptor (IP3R) through the molecular chaperone glucose-regulated protein 75 (grp75) and functional interaction between the channels was shown by the recombinant expression of the ligand-binding domain of the IP3R on the ER or mitochondrial surface.

TL;DR: Optimized protocols to isolate mitochondria-associated membranes, containing unique regions of ER membranes attached to the outer mitochondrial membrane and mitochondria without contamination from other organelles are provided.

TL;DR: The involvement of ROS and mitochondria in different molecular pathways controlling lifespan will be reported, pointing out the role of ROS as a “balance of power,” directing the cell towards life or death.

TL;DR: It is demonstrated that the regulated process of mitochondrial fusion and fission controls the spatiotemporal properties of mitochondrial Ca2+ responses and, thus, physiological and pathological consequences of cellularCa2+ signals.

TL;DR: It is demonstrated that protein kinase C β, activated by oxidative conditions in the cell, induces phosphorylation of p66Shc and triggers mitochondrial accumulation of the protein after it is recognized by the prolyl isomerase Pin1.