Eilert Hustede

TL;DR: Molecular data will be shown for genes of Alcaligenes eutrophus, purple non-sulfur bacteria, Such as Rhodospirillum rubrum, purple sulfur bacteria, such as Chromatium vinosum, pseudomonads belonging to rRNA homology group I, and for the Gram-positive bacterium Rhodococcus ruber.

TL;DR: Among 15 strains tested, only Chromatium vinosum strain 1611, C. purpuratum strain BN5500 and Lamprocystis roseopersicina strain 3112 were able to synthesize polyesters containing 3HV with propionate, valerate or heptanoate as carbon source.

TL;DR: Wild type cells of Rhodobacter sphaeroides and Rhodospirillum rubrum strains Ha and S1 as well as mutant cells defective in the synthesis of poly-(3-Hydroxybutyric acid) (PHB), were used to study the competition between PHB accumulation and photoproduction of hydrogen for reducing equivalents.

TL;DR: Both fragments hybridized with the PHB synthase structural gene of A. eutrophus H16 and conferred the ability to express PHB synthesis activity, and restored the ability of poly(3-hydroxybutyrate) (PHB) to accumulate in PHB-negative mutants derived from wild-type R. rubrum or R. sphaeroides.

TL;DR: Enzymatic analysis, nucleotide sequence analysis, hybridization studies and analysis of Tn5-induced PHA-negative mutants provided multiple evidence that phaCRs represents a monocistronic operon and that phACRs is isolated in the genome from other genes known to be relevant for PHA metabolism.