E
Ekaterina Savitskaya
Researcher at Skolkovo Institute of Science and Technology
Publications - 39
Citations - 1201
Ekaterina Savitskaya is an academic researcher from Skolkovo Institute of Science and Technology. The author has contributed to research in topics: CRISPR & CRISPR interference. The author has an hindex of 19, co-authored 38 publications receiving 1043 citations. Previous affiliations of Ekaterina Savitskaya include Kurchatov Institute & University of Oslo.
Papers
More filters
Journal ArticleDOI
High-throughput analysis of type I-E CRISPR/Cas spacer acquisition in E. coli
Ekaterina Savitskaya,Ekaterina Semenova,Vladimir G. Dedkov,Anastasia Metlitskaya,Konstantin Severinov +4 more
TL;DR: Despite a strong preference for an AAG PAM during CRISPR adaptation, the AAG (and CTT) triplets do not appear to be avoided in known E. coli phages, indicating thatCRISPR/Cas systems may not have been a strong factor in shaping host-virus interactions.
Journal ArticleDOI
Highly efficient primed spacer acquisition from targets destroyed by the Escherichia coli type I-E CRISPR-Cas interfering complex
Ekaterina Semenova,Ekaterina Savitskaya,Olga Musharova,Alexandra Strotskaya,Daria Vorontsova,Kirill A. Datsenko,Maria D. Logacheva,Konstantin Severinov +7 more
TL;DR: The result indicates that different functional outcomes of CRISPR-Cas response to two kinds of protospacer are not caused by different structures formed by the effector–crRNA complex but are due to the more rapid destruction of targets with fully matching protospacers.
Journal ArticleDOI
Foreign DNA acquisition by the I-F CRISPR-Cas system requires all components of the interference machinery.
Daria Vorontsova,Kirill A. Datsenko,Sofia Medvedeva,Joseph Bondy-Denomy,Ekaterina Savitskaya,Ksenia Pougach,Maria D. Logacheva,Blake Wiedenheft,Alan R. Davidson,Konstantin Severinov,Ekaterina Semenova +10 more
TL;DR: Spacer acquisition by a type I-F CRISPR–Cas system is studied and primed adaptation is observed, showing a gradient of acquisition efficiency as a function of distance from the priming site and a strand bias that is consistent with existence of single-stranded adaption intermediates.
Journal ArticleDOI
An endogenous Su(Hw) insulator separates the yellow gene from the Achaete-scute gene complex in Drosophila.
Anton Golovnin,Inna Biryukova,Inna Biryukova,Olga Romanova,Margarita Silicheva,Akeksander Parshikov,Ekaterina Savitskaya,Vincenzo Pirrotta,Pavel Georgiev +8 more
TL;DR: Genetic analysis shows that at least two proteins, Suppressor of Hairy wing and Modifier of MDG4, required for the activity of this insulator, are involved in the transcriptional regulation of Achaete-scute.
Journal ArticleDOI
PRE-mediated bypass of two Su(Hw) insulators targets PcG proteins to a downstream promoter.
Itys Comet,Ekaterina Savitskaya,Bernd Schuettengruber,Nicolas Nègre,Sergey Lavrov,Aleksander Parshikov,François Juge,E. M. Gracheva,Pavel Georgiev,Giacomo Cavalli +9 more
TL;DR: It is shown that PRE-dependent silencing is compatible with looping of the PRE in order to bring PcG proteins in contact with the promoter and does not require the coating of the whole chromatin domain between PRE and promoter.