J
Joseph Bondy-Denomy
Researcher at University of California, San Francisco
Publications - 94
Citations - 6442
Joseph Bondy-Denomy is an academic researcher from University of California, San Francisco. The author has contributed to research in topics: CRISPR & Bacteriophage. The author has an hindex of 32, co-authored 73 publications receiving 4508 citations. Previous affiliations of Joseph Bondy-Denomy include University of Toronto & University of California, Berkeley.
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Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system
TL;DR: The first examples of genes that mediate the inhibition of a CRISPR/Cas system are described, found in the genomes of bacteriophages infecting Pseudomonas aeruginosa and provides new insight into the co-evolution of phages and bacteria.
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Inhibition of CRISPR-Cas9 with Bacteriophage Proteins
Benjamin J. Rauch,Melanie R. Silvis,Judd F. Hultquist,Judd F. Hultquist,Christopher S. Waters,Michael J. McGregor,Michael J. McGregor,Nevan J. Krogan,Nevan J. Krogan,Joseph Bondy-Denomy +9 more
TL;DR: Four unique type II-A CRISPR-Cas9 inhibitor proteins encoded by Listeria monocytogenes prophages present tools that can be used to regulate the genome engineering activities of CRISpr-Cas 9.
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Multiple mechanisms for CRISPR–Cas inhibition by anti-CRISPR proteins
Joseph Bondy-Denomy,Bianca Garcia,Scott Strum,Mingjian Du,MaryClare F. Rollins,Yurima Hidalgo-Reyes,Blake Wiedenheft,Karen L. Maxwell,Alan R. Davidson +8 more
TL;DR: The first examples of proteins produced by phages that inhibit a CRISPR–Cas system are identified, and the diverse sequences and mechanisms of action of these anti-CRISPR proteins imply an independent evolution, and foreshadow the existence of other means by which proteins may alter CRISpr–Cas function.
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Disabling Cas9 by an anti-CRISPR DNA mimic
Jiyung Shin,Fuguo Jiang,Fuguo Jiang,Jun-Jie Liu,Jun-Jie Liu,Nicolas Bray,Benjamin J. Rauch,Seung Hyun Baik,Eva Nogales,Eva Nogales,Joseph Bondy-Denomy,Jacob E. Corn,Jennifer A. Doudna +12 more
TL;DR: It is shown that the anti-CRISPR protein AcrIIA4 binds only to assembled Cas9–single-guide RNA (sgRNA) complexes and not to Cas9 protein alone, demonstrating that inhibitors can modulate the extent and outcomes of Cas9-mediated gene editing.
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The CRISPR/Cas Adaptive Immune System of Pseudomonas aeruginosa Mediates Resistance to Naturally Occurring and Engineered Phages
TL;DR: These phages are only the second identified group of naturally occurring phages demonstrated to be blocked for replication by a nonengineered CRISPR/Cas system, and the results provide the first evidence that the P. aeruginosa type I-F CRIS PR-Cas system can function in phage resistance.