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Eric M. Phizicky
Researcher at University of Rochester
Publications - 111
Citations - 9880
Eric M. Phizicky is an academic researcher from University of Rochester. The author has contributed to research in topics: Transfer RNA & Saccharomyces cerevisiae. The author has an hindex of 49, co-authored 107 publications receiving 8944 citations. Previous affiliations of Eric M. Phizicky include Polish Academy of Sciences & Penn State Milton S. Hershey Medical Center.
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Journal ArticleDOI
Protein-protein interactions: methods for detection and analysis.
Eric M. Phizicky,Stanley Fields +1 more
TL;DR: This review describes how binding affinities can be evaluated by techniques including protein affinity chromatography, sedimentation, gel filtration, fluorescence methods, solid-phase sampling of equilibrium solutions, and surface plasmon resonance.
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tRNA biology charges to the front
Eric M. Phizicky,Anita K. Hopper +1 more
TL;DR: This review highlights new findings on the diverse pathways of tRNA maturation, and on the formation and function of a number of modifications, on the regulation of t RNA biosynthesis, quality control tRNA turnover mechanisms, widespread tRNA cleavage pathways activated in response to stress and other growth conditions.
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Protein analysis on a proteomic scale.
TL;DR: This work has shown that the generation of sets of clones that express a representative of each protein of a proteome in a useful format followed by the analysis of these sets on a genome-wide basis enables genetic, biochemical and cell biological technologies to be applied on a systematic level.
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Biochemical and genetic analysis of the yeast proteome with a movable ORF collection
Daniel M. Gelperin,Michael A. White,Martha L. Wilkinson,Yoshiko Kon,Li A. Kung,Kevin J. Wise,Nelson Lopez-Hoyo,Lixia Jiang,Stacy Piccirillo,Haiyuan Yu,Mark Gerstein,Mark E. Dumont,Eric M. Phizicky,Michael Snyder,Elizabeth J. Grayhack +14 more
TL;DR: A MORF (moveable ORF) library of 5854 yeast expression plasmids was constructed, each expressing a sequence-verified ORF as a C-terminal ORF fusion protein, under regulated control, demonstrating that nearly all verified ORFs are expressed and suggesting the authenticity of 48 ORFs characterized as dubious.
Journal ArticleDOI
A biochemical genomics approach for identifying genes by the activity of their products.
Mark R. Martzen,Stephen M. McCraith,Sherry L. Spinelli,Francy M. Torres,Stanley Fields,Elizabeth J. Grayhack,Eric M. Phizicky +6 more
TL;DR: Three previously unknown ORF-associated activities were identified: a cyclic phosphodiesterase that acts on adenosine diphosphate-ribose 1"-2" cyclic phosphate (Appr>p), an Appr-1"-p-processing activity, and a cytochrome c methyltransferase.