Lixia Jiang

TL;DR: A MORF (moveable ORF) library of 5854 yeast expression plasmids was constructed, each expressing a sequence-verified ORF as a C-terminal ORF fusion protein, under regulated control, demonstrating that nearly all verified ORFs are expressed and suggesting the authenticity of 48 ORFs characterized as dubious.

TL;DR: Examination of the binding targets of three related HOX factors--LIN-39, MAB-5, and EGL-5--indicates that these factors regulate genes involved in cellular migration, neuronal function, and vulval differentiation, consistent with their known roles in these developmental processes.

TL;DR: The results suggest that gene-regulatory properties previously observed for individual factors are general principles of metazoan regulation that are remarkably well-preserved despite extensive functional divergence of individual network connections.

TL;DR: In this article, the genome-wide binding locations of 165 human, 93 worm and 52 fly transcription regulatory factors were mapped for a total of 1,019 data sets from diverse cell types, developmental stages, or conditions in the three species, of which 498 (48.9%) are presented here for the first time.

TL;DR: This work determined the genomic distribution of binding sites for 92 transcription factors and regulatory proteins across multiple stages of Caenorhabditis elegans development by performing 241 ChIP-seq (chromatin immunoprecipitation followed by sequencing) experiments and produced a spatiotemporally resolved metazoan transcription factor binding map.