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Eva Sebestova
Researcher at Masaryk University
Publications - 15
Citations - 676
Eva Sebestova is an academic researcher from Masaryk University. The author has contributed to research in topics: Haloalkane dehalogenase & Dehalogenase. The author has an hindex of 11, co-authored 15 publications receiving 509 citations.
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Journal ArticleDOI
FireProt: Energy- and Evolution-Based Computational Design of Thermostable Multiple-Point Mutants.
David Bednar,Koen Beerens,Eva Sebestova,Jaroslav Bendl,Sagar D. Khare,Radka Chaloupková,Zbynek Prokop,Jan Brezovsky,David Baker,Jiri Damborsky +9 more
TL;DR: FireProt is a robust computational strategy for predicting highly stable multiple-point mutants that combines energy- and evolution-based approaches with smart filtering to identify additive stabilizing mutations and can be applied to any protein for which a tertiary structure and homologous sequences are available.
Journal ArticleDOI
Engineering enzyme stability and resistance to an organic cosolvent by modification of residues in the access tunnel
Tana Koudelakova,Radka Chaloupková,Jan Brezovsky,Zbynek Prokop,Eva Sebestova,Martin Hesseler,Morteza Khabiri,Maryia Plevaka,Daryna Kulik,Ivana Kuta Smatanova,Ivana Kuta Smatanova,Pavlina Rezacova,Rüdiger Ettrich,Uwe T. Bornscheuer,Jiri Damborsky +14 more
TL;DR: Protein crystallography and molecular dynamics revealed that the tunnel residue packing is a key determinant of protein stability and the active-site accessibility for co-solvent molecules.
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CAVER: algorithms for analyzing dynamics of tunnels in macromolecules
TL;DR: The principles of the CAVER 3.0 algorithms for the identification and analysis of properties of transport pathways both in static and dynamic structures are described and the improved clustering solution for finding tunnels in macromolecules is introduced.
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HotSpot Wizard 2.0: automated design of site-specific mutations and smart libraries in protein engineering
Jaroslav Bendl,Jan Stourac,Eva Sebestova,Ondrej Vavra,Milos Musil,Jan Brezovsky,Jiri Damborsky +6 more
TL;DR: Overall, HotSpot Wizard provides comprehensive annotations of protein structures and assists protein engineers with the rational design of site-specific mutations and focused libraries.
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Exploration of Enzyme Diversity by Integrating Bioinformatics with Expression Analysis and Biochemical Characterization
Pavel Vanacek,Eva Sebestova,Petra Babková,Šárka Bidmanová,Lukas Daniel,Pavel Dvorak,Veronika Stepankova,Radka Chaloupková,Jan Brezovsky,Zbynek Prokop,Jiri Damborsky +10 more
TL;DR: An integrated system for automated in silico screening and systematic characterization of diverse family members of genetically unrelated Bacteria, Eukaryota, and also A...