Fan Bai

TL;DR: The number and rapid turnover of protein subunits within a functioning molecular machine are measured with single-molecule precision in functioning bacterial flagellar motors in Escherichia coli for the first time.

TL;DR: The LAR subtype showed more ERBB2 somatic mutations, infrequent mutational signature 3 and frequent CDKN2A loss than the other subtypes, and was classified as a mesenchymal-like subtype.

TL;DR: The single-cell transcriptional landscape of moderate, severe and convalescent cases of patients with COVID-19 describes the dynamic nature of immune responses during disease progression, and shows a deranged interferon response, profound immune exhaustion with skewed T cell receptor repertoire and broad T cell expansion.

TL;DR: Experimental and computational analyses identify multiple cell populations during the epithelial-to-mesenchymal transition and provide experimental confirmation for a model of cascading switches in phenotypes associated with TGF-β1–induced EMT of MCF10A cells that involves two double-negative feedback loops.

TL;DR: The use of the recently developed multiple annealing and looping-based amplification cycles for whole-genome amplification of single CTCs from lung cancer patients are reported, finding that copy number variations (CNVs), one of the major genomic variations, are specific to cancer types, reproducible from cell to cell, and even from patient to patient.