http://www.bio21.bas.bg/ibf/PC_review.pdf

Phases and phase transitions of the phosphatidylcholines

Dielectric Relaxation and Solvation Dynamics of Water in Complex Chemical and Biological Systems

Mixtures of long-chain and short-chain phosphatidylcholine (PC) were characterized by multinuclear (13C, 31P, 2H) solid-state nuclear magnetic resonance. This work complements and extends previous characterization of such mixtures by focusing on concentrated mixtures at temperatures above the gel to liquid crystalline phase transition temperature (Tm) of the long-chain PC component. Above Tm it was observed that highly oriented, bilayer-like assemblies could be formed of mixtures of dimyristoylphosphatidylcholine (DMPC) and dihexanoylphosphatidylcholine (DHPC) in molar ratios ranging from approximately 1:3.5 to 1:2 (DHPC:DMPC) over a considerable range of lipid concentrations (at least 3-40% w/v total lipid, for a 1:2.5 sample). Orientation was observed to occur only in an L alpha-like phase. The NMR data can be accounted for by a general model of the DHPC-DMPC aggregates in which DHPC can be found in two distinct populations (one highly ordered, one not). The averaged conformations of the glycerol backbone/headgroup regions of the long- and short-chain PC composing the assemblies were judged by solid-state 13C NMR to be similar to each other. The information gleaned about these mixtures and the quality of the oriented NMR spectra obtained suggest that DHPC-DMPC mixtures may prove to be useful as model membrane media in solid-state NMR studies of biomembranes.

http://www.cs.duke.edu/brd/Teaching/Bio/asmb/Papers/NMR/Residual-dipolar-coupling/Spinning/bi00152a029.pdf

Characterization of magnetically orientable bilayers in mixtures of dihexanoylphosphatidylcholine and dimyristoylphosphatidylcholine by solid-state NMR.

Magnetically-oriented phospholipid micelles as a tool for the study of membrane-associated molecules

Phospholipid head groups as sensors of electric charge in membranes.

Magnetic ordering of phospholipid membranes

Hydration of Escherichia coli lipids. Deuterium T1 relaxation time studies of phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine

Ca2+ binding to phosphatidylglycerol bilayers as studied by differential scanning calorimetry and 2H- and 31P-nuclear magnetic resonance

Comparative 2H- and 31P-NMR study on the properties of palmitoyllysophosphatidylcholine in bilayers with gramicidin, cholesterol and dipalmitoylphosphatidylcholine

François Borle

Papers

Magnetic ordering of phospholipid membranes

Hydration of Escherichia coli lipids. Deuterium T1 relaxation time studies of phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine

Ca2+ binding to phosphatidylglycerol bilayers as studied by differential scanning calorimetry and 2H- and 31P-nuclear magnetic resonance

Comparative 2H- and 31P-NMR study on the properties of palmitoyllysophosphatidylcholine in bilayers with gramicidin, cholesterol and dipalmitoylphosphatidylcholine

Structure of Escherichia coli membranes. Deuterium magnetic resonance studies of the phosphoglycerol head group in intact cells and model membranes