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Showing papers in "Biochimica et Biophysica Acta in 1985"


Journal ArticleDOI
TL;DR: In this paper, the electron transfer reactions between ions and molecules in solution have been the subject of considerable experimental study during the past three decades, including charge transfer, photoelectric emission spectra, chemiluminescent electron transfer, and electron transfer through frozen media.

7,155 citations


Journal ArticleDOI
TL;DR: A technique for the rapid production of large unilamellar vesicles by repeated extrusion under moderate pressures by employing the lipophilic cation methyltriphenylphosphonium (MTPP⁺) and LUVET systems exhibiting a membrane potential in response to a transmembrane Na⁺/K⁺ gradient have been characterized.

2,092 citations


Journal ArticleDOI
TL;DR: Recent studies concerning cholesterol, its behavior and its roles in cell growth provide important new clues to the role of this fascinating molecule in normal and pathological states.

1,457 citations


Journal ArticleDOI
TL;DR: Metallothionein has the characteristics of a sacrificial but renewable cellular target for .OH-mediated cellular damage, and appears to be an extraordinarily efficient .OH radical scavenger even when compared to proteins 10-50-times its molecular weight.

1,016 citations


Journal ArticleDOI
TL;DR: The lipid content and lipid composition of mitochondria, a comparison between mitochondrial and other cellular membranes, and mitocbondria from microorganisms are compared.

939 citations


Journal ArticleDOI
TL;DR: The similarity of amino acid composition, Se content and kinetic mechanism, relative to the difference in substrate specificity, indicates that the two enzymes 'classical' glutathione peroxidase and phospholipid hydroperoxide glutathions are in some way related.

897 citations


Journal ArticleDOI
TL;DR: Discriminant analysis can be used to precisely classify membrane proteins as integral or peripheral and to estimate the odds that the classification is correct, and it is found that discrimination between integral and peripheral membrane proteins can be achieved with 99% reliability.

819 citations





Journal ArticleDOI
TL;DR: It is demonstrated that egg phosphatidylcholine MLV systems dispersed in the presence of Mn2+ also exhibit non-equilibrium solute distributions, and that repetitive freeze-thawing cycles can remove such solute heterogeneity.


Journal ArticleDOI
TL;DR: Research into the mechanism and function of plasminogen activation, and the role of fibrin in the regulation of activator secretion, are presented.

Journal ArticleDOI
TL;DR: It is concluded that the reactions of hypochlorite accurately reflect those of the myeloperoxidase system and the significance of these reactions in microbial killing and inflammation is discussed.


Journal ArticleDOI
TL;DR: Thermally labile azo-initiators used to generate peroxyl radicals at a known, steady rate in an aqueous dispersion of dilinoleoylphosphatidylcholine multilamellar liposomes are used to study the antioxidant behaviour of asCorbate itself and ascorbate in combination with a water-soluble alpha-tocopherol analogue (TROLOX(-].

Journal ArticleDOI
TL;DR: Analysis of existing data suggests that within experimental error both these distinctively different approaches are valid, indicating that the critical effective ratio at which solubilization occurs is approximately equal to the product of the critical micelle concentration and the distribution coefficient K.


Journal ArticleDOI
TL;DR: An intact organelle, the prostasome, is secreted by the acinar epithelial cell of the human prostate gland and may be important for promoting forward motility of spermatozoa.

Journal ArticleDOI
TL;DR: A cytochrome c-551 and a pair of 'high redox-potential' ferredoxins (iso-high-potentially iron-sulfur proteins) were found to be the major soluble electron-transport proteins in Ectothiorhodospira halophila.

Journal ArticleDOI
TL;DR: It is suggested that Al( III) ions produce an alteration in membrane structure that facilitates lipid peroxidation, and that the increased formation of fluorescent age pigments in the nervous system of patients exposed to toxic amounts of Al(III) may be related to this phenomenon.

Journal ArticleDOI
TL;DR: The hypothesis that increased free-radical activity may play some role in muscle damage caused by extensive muscular activity is supported.


Journal ArticleDOI
TL;DR: The effects of various stilbenes (i.e., 3,4,5-trihydroxystilbene, 3, 4,5,trihydroxylstilbinene 3-O-d -glucoside and 2,3,4',5-tetrahydroxystielbene 2-O-, d-glucose) isolated from the roots of Polygonum species on rat peritoneal polymorphonuclear leukocyte lipoxygenase and cycloencogenase activities were studied in this paper


Journal ArticleDOI
TL;DR: It was found that vesicle size and surface charge override state of membrane permeability in determining rates of vesicular clearance, and half-lives of liposomes that were practically impermeable in plasma were much shorter for larger vesicles or vESicles with a negative charge on their surface.

Journal ArticleDOI
TL;DR: The critical domain role for sterol is observed under conditions of lanosterol supplementation where low levels of ergosterol (10-times those necessary for sparking on cholestanol) are required for growth.

Journal ArticleDOI
TL;DR: Heat inactivation of oxygen evolution by isolated Photosystem II particles was accelerated by Cl − depletion and exogenous Mn 2+ .

Journal ArticleDOI
TL;DR: In this article, the temperature dependence of electron transfer to ubiquinone from the bacteriopheophytin (BPh) that serves as an initial electron acceptor (I) in reaction centers of Rhodopseudomonas sphaeroides was examined.

Journal ArticleDOI
TL;DR: In this article, a theoretical model of the monolayer fusion is suggested to explain the results, assuming that the structural reorganization underlying the process involves the formation of a stalk between bilayers as a result of local bending of the interacting monolayers.