G. Clérico

TL;DR: It is shown for the first time that in the horse, as in other species, intracellular reactive oxygen species are increased by the process of vitrification and melatonin was useful in reducing mitochondrial-related ROS and improving ICSI embryo development, although the lower pregnancy rate in presence of melatonin should be further analyzed in future studies.

TL;DR: This study indicates it is possible to use a 14-day interval for oocyte collection in water buffalo; this approach could be considered as an alternative when access to animals is restricted.

TL;DR: The maturation dynamics of equine oocytes at different time points, from 20 to 40h (Experiment 1), and equine ICSI blastocysts were produced from oocytes that exhibited early or late extrusion of the first polar body (PB), showing an improvement in total cell number and blastocyst rate.

TL;DR: In this article , a new method for sex determination using leakage from manual collapse prior to vitrification was investigated, a technique which previously resulted in a pregnancy in our laboratory (1 of 3, unpublished results) and hypothesized that leaked blastocele fluid into the surrounding medium could provide sufficient DNA for amplification and sex determination.