G. E. Brown

TL;DR: Reovirus messenger RNAs are degraded faster in crude extracts from mouse Ehrlich ascites tumor cells which have been treated with either a partially purified interferon preparation or the interferons inducer poly(I)·poly(C) than in corresponding extracts from untreated cells.

TL;DR: Characteristics of the faster degradation of reovirus mRNA in S30INT supplemented with dsRNA are described and it is shown that ATP is required, in addition to ds RNA, for the acceleration of mRNA degradation in S 30INT, and this phenomenon can be divided into two phases.

TL;DR: It is found that the rate of inactivation of some tRNA's but not those of many others is faster in S30INT than in corresponding extracts from control cells, and the relationship of the increased rate of tRNA inactivation to the antiviral effect of interferon is unclear.

TL;DR: After the methylation of unmethylated reovirus mRNA (reo mRNAU) has stopped in S30INT, the RNA can be reisolated and further methylated in an extract of control cells (S30C), indicating that the impairment of methylation in S 30INT cannot be due to cleavage or irreversible inactivation of reo RNAU.

TL;DR: The methylation of (unmethylated) reovirus mRNA is impaired in extracts of interferon-treated Ehrlich ascites tumor cells, and it is conceivable that the impairment of methylation may be part of the antiviral activities ofinterferons.