G
Galina A. Stepanyuk
Researcher at Russian Academy of Sciences
Publications - 22
Citations - 620
Galina A. Stepanyuk is an academic researcher from Russian Academy of Sciences. The author has contributed to research in topics: Coelenterazine & Photoprotein. The author has an hindex of 14, co-authored 22 publications receiving 527 citations. Previous affiliations of Galina A. Stepanyuk include Bayer Corporation & Scripps Research Institute.
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Journal ArticleDOI
Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state
Zhi-Jie Liu,Galina A. Stepanyuk,Eugene S. Vysotski,John G. Lee,Svetlana V. Markova,Natalia P. Malikova,Bi-Cheng Wang +6 more
TL;DR: From such a higher energy state it is now energetically feasible to account for the shorter wavelength bioluminescence spectra obtained from some photoprotein mutants or to populate the lower energy state of the phenolate anion to yield the blue biolUMinescence ordinarily observed from native photoproteins.
Journal ArticleDOI
Interchange of aequorin and obelin bioluminescence color is determined by substitution of one active site residue of each photoprotein
Galina A. Stepanyuk,Stefan Golz,Svetlana V. Markova,Svetlana V. Markova,Ludmila A. Frank,John Lee,Eugene S. Vysotski,Eugene S. Vysotski +7 more
TL;DR: All mutants were stable with good activity and were expressible in mammalian cells, thereby demonstrating potential for monitoring multiple events in cells using multi‐color detection.
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Transient Protein-RNA Interactions Guide Nascent Ribosomal RNA Folding.
TL;DR: It is found that co-transcriptional rRNA folding is complicated by the formation of long-range RNA interactions and that r-proteins self-chaperone the r RNA folding process prior to stable incorporation into a ribonucleoprotein (RNP) complex.
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NMR derived topology of a GFP-photoprotein energy transfer complex
Maxim S. Titushin,Yingang Feng,Galina A. Stepanyuk,Galina A. Stepanyuk,Yang Li,Svetlana V. Markova,Stefan Golz,Bi-Cheng Wang,John Lee,Jinfeng Wang,Eugene S. Vysotski,Zhi-Jie Liu +11 more
TL;DR: A spatial structure of a complex of the Ca2+-regulated photoprotein clytin with its green-fluorescent protein (cgGFP) from the jellyfish Clytia gregaria is presented, and it is shown that it accounts for the bioluminescence properties of this system in vitro.
Journal ArticleDOI
Spectral tuning of obelin bioluminescence by mutations of Trp92
Natalia P. Malikova,Galina A. Stepanyuk,Ludmila A. Frank,Svetlana V. Markova,Svetlana V. Markova,Eugene S. Vysotski,Eugene S. Vysotski,John Lee +7 more
TL;DR: The Ca2+‐regulated photoprotein obelin was substituted at Trp92 by His, Lys, Glu, and Arg and all mutants fold into stable conformations and produce bimodal bioluminescence spectra with enhanced contribution from a violet emission.