George Hedger

TL;DR: The results clarify the structural mechanism by which the activity of a GPCR is controlled by ligand-regulated interactions between its extracellular and transmembrane domains.

TL;DR: In this paper, the authors used native mass spectrometry to identify endogenous lipids bound to three class A GPCRs and found that PtdIns(4,5)P2 was preferential binding over related lipids.

TL;DR: Recent notable biomolecular simulation studies which have identified lipid interaction sites on a range of different membrane proteins agree well with those identified by complementary experimental techniques, demonstrating the power of the molecular dynamics approach in the prediction and characterization of lipid interaction Sites on integral membrane proteins.

TL;DR: The use of replica-exchange umbrella sampling starting from different initial conditions as a robust approach for calculation of the binding energies in membrane simulations is proposed, revealing previously unnoticed complexities of the dimerization free energy landscape.

TL;DR: The results demonstrate that the juxtamembrane (JM) regions of RTKs are critical for inducing clustering of anionic lipids, including PIP2, both in simple asymmetric bilayers, and in more complex mixed membranes.