The influence of diet on the distribution of nitrogen isotopes in animals was investigated by analyzing animals grown in the laboratory on diets of constant nitrogen isotopic composition. 
The isotopic composition of the nitrogen in an animal reflects the nitrogen isotopic composition of its diet. The δ^(15)N values of the whole bodies of animals are usually more positive than those of their diets. Different individuals of a species raised on the same diet can have significantly different δ^(15)N values. The variability of the relationship between the δ^(15)N values of animals and their diets is greater for different species raised on the same diet than for the same species raised on different diets. Different tissues of mice are also enriched in ^(15)N relative to the diet, with the difference between the δ^(15)N values of a tissue and the diet depending on both the kind of tissue and the diet involved. The δ^(15)N values of collagen and chitin, biochemical components that are often preserved in fossil animal remains, are also related to the δ^(15)N value of the diet. 
The dependence of the δ^(15)N values of whole animals and their tissues and biochemical components on the δ^(15)N value of diet indicates that the isotopic composition of animal nitrogen can be used to obtain information about an animal's diet if its potential food sources had different δ^(15)N values. The nitrogen isotopic method of dietary analysis probably can be used to estimate the relative use of legumes vs non-legumes or of aquatic vs terrestrial organisms as food sources for extant and fossil animals. However, the method probably will not be applicable in those modern ecosystems in which the use of chemical fertilizers has influenced the distribution of nitrogen isotopes in food sources. 
The isotopic method of dietary analysis was used to reconstruct changes in the diet of the human population that occupied the Tehuacan Valley of Mexico over a 7000 yr span. Variations in the δ^(15)C and δ^(15)N values of bone collagen suggest that C_4 and/or CAM plants (presumably mostly corn) and legumes (presumably mostly beans) were introduced into the diet much earlier than suggested by conventional archaeological analysis.

Influence of Diet On the Distribtion of Nitrogen Isotopes in Animals

The post-genomics era has brought with it ever increasing demands to observe and characterise variation within biological systems. This variation has been studied at the genomic (gene function), proteomic (protein regulation) and the metabolomic (small molecular weight metabolite) levels. Whilst genomics and proteomics are generally studied using microarrays (genomics) and 2D-gels or mass spectrometry (proteomics), the technique of choice is less obvious in the area of metabolomics. Much work has been published employing mass spectrometry, NMR spectroscopy and vibrational spectroscopic techniques, amongst others, for the study of variations within the metabolome in many animal, plant and microbial systems. This review discusses the advantages and disadvantages of each technique, putting the current status of the field of metabolomics in context, and providing examples of applications for each technique employed.

Measuring the metabolome: current analytical technologies

Near infrared reflectance spectroscopy (NIRS) is a non-destructive and rapid technique applied increasingly for food quality evaluation in recent years. It provides us more information to research the quality of food products. This review intends to give an overview of the type of information that can be obtained based on some developed theory and food research of NIRS. It includes the principle of NIRS technique, the specific techniques with chemometrics for data pre-processing methods, qualitative and quantitative analysis and model transfer, and the wide applications of NIRS in food science. In addition, the promise of NIRS technique for food quality evaluation is demonstrated, and some problems which need to be solved or investigated further are also discussed.

Theory and application of near infrared reflectance spectroscopy in determination of food quality

Spore-forming bacilli are being explored for the production and preservation of food for many centuries. The inherent ability of production of large number of secretory proteins, enzymes, antimicrobial compounds, vitamins, and carotenoids specifies the importance of bacilli in food chain. Additionally, Bacillus spp. are gaining interest in human health related functional food research coupled with their enhanced tolerance and survivability under hostile environment of gastrointestinal tract (GIT). Besides, bacilli are more stable during processing and storage of food and pharmaceutical preparations, making them more suitable candidate for health promoting formulations. Further, Bacillus strains also possess biotherapeutic potential which is connected with their ability to interact with the internal milieu of the host by producing variety of antimicrobial peptides and small extracellular effector molecules. Nonetheless, with proposed scientific evidences, commercial probiotic supplements and functional foods comprising of Bacillus spp. had not gained much credential in general population, since the debate over probiotic vs pathogen tag of Bacillus in the research and production terrains is confusing consumers. Hence, it’s important to clearly understand the phenotypic and genotypic characteristics of selective beneficial Bacillus spp. and their substantiation with those having GRAS status, to reach a consensus over the same. This review highlights the probiotic candidature of spore forming Bacillus spp. and presents an overview of the proposed health benefits, including application in food and pharmaceutical industry. Moreover, the growing need to evaluate the safety of individual Bacillus strains as well as species on a case by case basis and necessity of more profound analysis for the selection and identification of Bacillus probiotic candidates are also taken into consideration.

/pdf/bacillus-as-potential-probiotics-status-concerns-and-future-2dfafhgjyh.pdf

Bacillus As Potential Probiotics: Status, Concerns, and Future Perspectives.

The Olive tree (Olea europaea L), a native of the Mediterranean basin and parts of Asia, is now widely cultivated in many other parts of the world for production of olive oil and table olives Olive is a rich source of valuable nutrients and bioactives of medicinal and therapeutic interest Olive fruit contains appreciable concentration, 1–3% of fresh pulp weight, of hydrophilic (phenolic acids, phenolic alchohols, flavonoids and secoiridoids) and lipophilic (cresols) phenolic compounds that are known to possess multiple biological activities such as antioxidant, anticarcinogenic, antiinflammatory, antimicrobial, antihypertensive, antidyslipidemic, cardiotonic, laxative, and antiplatelet Other important compounds present in olive fruit are pectin, organic acids, and pigments Virgin olive oil (VOO), extracted mechanically from the fruit, is also very popular for its nutritive and health-promoting potential, especially against cardiovascular disorders due to the presence of high levels of monounsaturates and other valuable minor components such as phenolics, phytosterols, tocopherols, carotenoids, chlorophyll and squalene The cultivar, area of production, harvest time, and the processing techniques employed are some of the factors shown to influence the composition of olive fruit and olive oil This review focuses comprehensively on the nutrients and high-value bioactives profile as well as medicinal and functional aspects of different parts of olives and its byproducts Various factors affecting the composition of this food commodity of medicinal value are also discussed

https://www.mdpi.com/1422-0067/13/3/3291/pdf

Valuable Nutrients and Functional Bioactives in Different Parts of Olive (Olea europaea L.)—A Review

Ce texte a pour objet de decrire une methode standardisee de mesure de la digestibilite totale des aliments chez le lapin. Cette methode a ete elaboree en commun au sein du Groupe Europeen de Nutrition Cunicole (EGRAN) qui rassemble six laboratoires appartenant a cinq pays (Belgique, Espagne, France, ltalie et Portugal). Cette nouvelle methode a ete concue pour etre a la fois precise, rapide, et reproductible. Facilement applicable a l'ensemble des laboratoires, elle peut desormais servir de reference dans le secteur cunicole. Cette methode utilise des lapins de 7 a 8 semaines loges en cages individuelles et nourris a volonte. La duree de la periode pre-experimentale est de 7 jours et celle de la periode de recolte de 4 jours seulement. Les conditions standardisees d´echantillonnage et de sechage sont decrites pour l'aliment et les faeces.

https://polipapers.upv.es/index.php/wrs/article/download/239/226

European reference method for in vivo determination of diet digestibility in rabbits

Effect of genotype, gender and feed restriction on growth, meat quality and the occurrence of white striping and wooden breast in broiler chickens

The aim of this paper was to draw up a set of recommendations for applied nutrition and feeding trials with rabbits, in relation to certain aspects such as determining the nutritive value of raw materials or diets in growing or reproducing animals, studying digestive physiology and obtaining growth and reproduction parameters. We deal first with animals, size of the sample, housing conditions, diets, handling, measurements, and the data analyses relevant to the design of the experiment are described. Secondly, we give a list of recommended items and include some comments.

https://www.polipapers.upv.es/index.php/wrs/article/download/516/503

Recommendations and guidelines for applied nutrition experiments in rabbits.

Main force and weakness points of poultry and rabbit production chains are presented and meat quality discussed in relation to nutritional and technological issues. An analysis of the most important poultry and rabbit meat quality traits and their major relationships with production factors (genotype, feeding, housing, pre-slaughter handling, slaughtering, and processing) is provided. Most recent research advancements are presen- ted in view of the consumer’s demand for healthy and safe products obtained respecting animal welfare.

Advances in research on poultry and rabbit meat quality

Research on rabbit welfare has been fragmentary and to date has been performed by only a few European teams who have often neglected the productive and commercial aims of rabbit rearing. With European Project COST Action 848 (Multi-facetted research in rabbits: a model to develop healthy and safe production in respect with animal welfare), rabbit welfare began to be considered in European research projects with the focus mainly on ethology, welfare evaluation methods, doe-litter relationships, man-animal relationships, and reproducing and fattening housing systems. As regards legislation, since 1996 the Standing Committee for the protection of animals kept for farming purposes established in the European Council by the Convention on the Protection of farm animals (ETS 87/1976) has been preparing specific recommendations to ensure improved welfare of domestic rabbits kept for commercial purposes, which should provide the basis for future European and national regulations. The European Commission asked the European Food and Safety Authority (EFSA) for its opinion on "The impact of the current housing and husbandry systems on the health and welfare of farmed domestic rabbits". The present paper provides a critical review of the existing literature on rabbit welfare with special emphasis on housing conditions.

https://polipapers.upv.es/index.php/wrs/article/download/553/540

Gerolamo Xiccato

Papers

European reference method for in vivo determination of diet digestibility in rabbits

Effect of genotype, gender and feed restriction on growth, meat quality and the occurrence of white striping and wooden breast in broiler chickens

Recommendations and guidelines for applied nutrition experiments in rabbits.

Advances in research on poultry and rabbit meat quality

Animal welfare in reared rabbits: a review with emphasis on housing systems.