Gita Venkatakrishnan

TL;DR: Three important conclusions emerge from this study: internalization of NGFRs is not necessary for some early rapid transcriptional effects of NGF, an unknown factor(s) that cooperates with the cloned NGFR in allowing high-affinity NGF binding is found in a primitive central nervous system cell line, andNGFRs introduced into and expressed by D283 MED are unable to induce differentiation in these primitive neuron-like tumor cells.

TL;DR: Although CHP707m is the first central nervous system PNET cell line proven to express NGF receptors, immunohistological survey of tissue sections prepared from human central nervous System PNETs showed that 13 of 35 contained NGF receptor‐positive tumor cells, suggesting more than one‐third of such tumors might be responsive to the effects of NGF.

TL;DR: NGFR is preclustered and immobile on responsive cells, which suggests that immobilization of NGFR prior to ligand binding is required for signal transduction.

TL;DR: The number of high‐affinity NGF binding sites was nearly the same for LAN5 and LAN5/NGFR, a finding suggesting that there is a limiting number of some separately coded factor or subunit that is required for high-affinity NGFRs.

TL;DR: NGFr expressed on COS and A875 cells are diffusely distributed, but NGFr on the surface of PC12 cells appeared, for some cells, to be patched, and NGF had no effect on the diffusing fraction or the distribution of NGFR for any cell line.