Gökhan Tolun

TL;DR: The loading of Mcm2-7 onto DNA requires the origin recognition complex (ORC), Cdc6, and Cdt1, and depends on ATP, and has significant implications for understanding how eukaryotic DNA replication origins are chosen and licensed, how replisomes assemble during initiation, and how unwinding occurs during DNA replication.

TL;DR: It is concluded that minicircle replication initiation is likely EB's most vulnerable target, but the effect on nuclear replication may also contribute to cell killing.

TL;DR: Using electron microscopy, it is shown that both wild-type WRN and a helicase-defective mutant bind with exceptionally high specificity to DNA secondary structures at the replication fork and at Holliday junctions, little or no binding is observed elsewhere on the DNA molecules.

TL;DR: The unusual assembly of the Hermes transposase-DNA complex reveals that Hermes forms an octameric ring organized as a tetramer of dimers, which explains the basis of bipartite DNA recognition at hAT transposon ends, provides a rationale for transPOSon end asymmetry, and suggests how the avidity provided by multiple sites of interaction could allow a transpos enzyme to locate its transposons ends amidst a sea of chromosomal DNA.

TL;DR: A continuous DNase assay based on the differential fluorescence output of a DNA dye ligand called PicoGreen is described and appears to have general utility as it is also suitable for measuring the DNA digestion activities of a processive helicase/nuclease, RecBCD, a distributive exonuclease