Grant J. Steyer

TL;DR: A mouse, in which enhanced green fluorescent protein was expressed under gamma actin promoter in smooth muscle cells, gave clear 3D views of smooth muscle in the urogenital and gastrointestinal tracts, and cryo‐imaging could obtain 3D vasculature down to 10 μm, over very large regions of mouse brain.

TL;DR: A cryo-imaging system, which alternates between sectioning and imaging bright field and fluorescence block-face image volumes with micron-scale-resolution, and software for reduction of out-of-plane fluorescence is developed for applications requiring single-cell detection of fluorescently labeled cells anywhere in a mouse.

TL;DR: The Case cryo-imaging system is a section and image system which allows one to acquire micron-scale, information rich, whole mouse color bright field and molecular fluorescence images of an entire mouse, and the 3D results demonstrate the novel true-color volume visualization tools the team has developed.

TL;DR: Next-image was faster than other methods and less prone to image processing artifacts, and RL is recommended for the best restoration of the shape and size of fluorescent structures.

TL;DR: Multi-scale, live-time interactive visualization of color image data, including microscopic whole-mouse cryo-images serving many biomedical applications, and interactively, selectively enhanced anatomy using feature detection are developed.