G
Gunter B. Kohlhaw
Researcher at Purdue University
Publications - 45
Citations - 2134
Gunter B. Kohlhaw is an academic researcher from Purdue University. The author has contributed to research in topics: Leucine & Gene. The author has an hindex of 26, co-authored 45 publications receiving 2063 citations.
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Journal ArticleDOI
Leucine Biosynthesis in Fungi: Entering Metabolism through the Back Door
TL;DR: Branched-chain amino acid biosynthesis and its regulation in other fungi are summarized, the question of leucine as metabolic signal is addressed, and possible directions of future research in this area are outlined.
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Nucleotide sequence of yeast LEU2 shows 5'-noncoding region has sequences cognate to leucine
TL;DR: The LEU2 structural gene and its regulatory sequences were isolated on a 2200 bp Xho I-Sal I fragment as mentioned in this paper, where the leucine codon usage in this reading frame follows exactly that of other yeast genes.
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Yeast LEU2. Repression of mRNA levels by leucine and primary structure of the gene product.
TL;DR: It has been known that enzyme activity associated with the yeast LEU1 and LEU2 gene product (beta-isopropylmalate dehydrogenase) drops sharply when yeast is grown in the presence of leucine, and RNA blot hybridizations with LEu2-specific probes establish that this is accompanied by a 5-fold repression in LEU 2 mRNA levels.
Journal ArticleDOI
Subcellular Localization of Isoleucine-Valine Biosynthetic Enzymes in Yeast
E. D. Ryan,Gunter B. Kohlhaw +1 more
TL;DR: Density gradient centrifugation and the behavior of marker enzymes suggest that all of the above enzymes of the isoleucine-valine biosynthetic pathway are associated with the mitochondria.
Journal ArticleDOI
In Vitro Transcriptional Activation by a Metabolic Intermediate: Activation by Leu3 Depends on α-Isopropylmalate
TL;DR: An example in eukaryotes of direct transcriptional regulation by a small effector molecule is presented.