Subcellular Localization of Isoleucine-Valine Biosynthetic Enzymes in Yeast
E. D. Ryan,Gunter B. Kohlhaw +1 more
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TLDR
Density gradient centrifugation and the behavior of marker enzymes suggest that all of the above enzymes of the isoleucine-valine biosynthetic pathway are associated with the mitochondria.Abstract:
By using the method of stepwise homogenization of yeast spheroplast lysates employed previously with the leucine biosynthetic enzymes, it is shown that threonine deaminase, acetohydroxy acid synthase, Mg2+-dependent isomero-reductase, and dihydroxy acid dehydratase are particulate. Density gradient centrifugation and the behavior of marker enzymes suggest that all of the above enzymes of the isoleucine-valine biosynthetic pathway are associated with the mitochondria.read more
Citations
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Journal ArticleDOI
Enzymic analysis of the crabtree effect in glucose-limited chemostat cultures of Saccharomyces cerevisiae.
TL;DR: The physiology of Saccharomyces cerevisiae CBS 8066 was studied in glucose-limited chemostat cultures and it was concluded that the occurrence of alcoholic fermentation is not primarily due to a limited respiratory capacity, but rather, organic acids produced by the organism may have an uncoupling effect on its respiration.
Patent
Fermentive Production of Four Carbon Alcohols
TL;DR: In this article, a method for the fermentative production of four carbon alcohols is presented, butanol, preferably isobutanol, is produced by the fermentation of a recombinant bacterium expressing an isobutoanol biosynthetic pathway.
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The Development of Herbicide Resistant Crops
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TL;DR: Examples of the introduction of herbicides resistance into crop plants by several different techniques are presented, the status of development of herbicide-resistant cultivars, and future prospects for such work are presented.
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Leucine Biosynthesis in Fungi: Entering Metabolism through the Back Door
TL;DR: Branched-chain amino acid biosynthesis and its regulation in other fungi are summarized, the question of leucine as metabolic signal is addressed, and possible directions of future research in this area are outlined.
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Isolation and characterization of plant genes coding for acetolactate synthase, the target enzyme for two classes of herbicides.
TL;DR: The tobacco ALS gene was isolated from a line of tobacco which is resistant to the sulfonylurea herbicides due to an alteration in ALS, and codes for an ALS that is sensitive to the herbicides, as assayed by transformation of the gene into sensitive tobacco cells.
References
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[45] Cytochrome oxidase from beef heart mitochondria
TL;DR: This chapter discusses the cytochrome c oxidase from beef heart mitochondria, which is inhibited by cyanide, azide, hydroxylamine, and sodium sulfide.
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Glucose 6-phosphate dehydrogenase (Zwischenferment). I. Isolation of the crystalline enzyme from yeast.
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Cytoplasmic Type 80 S Ribosomes Associated with Yeast Mitochondria II. EVIDENCE FOR THE ASSOCIATION OF CYTOPLASMIC RIBOSOMES WITH THE OUTER MITOCHONDRIAL MEMBRANE IN SITU
TL;DR: Electron microscopic examination of growing spheroplasts prepared from Saccharomyces cerevisiae revealed ribosome-like particles of 150 A diameter aligned along the outer mitochondrial membrane, suggesting a role for the nascent polypeptide chains in the attachment of cytoplasmic polysomes to mitochondria.
Journal ArticleDOI
Assay of yeast enzymes in situ. A potential tool in regulation studies.
TL;DR: A permeabilization method which allows the assay of several intracellular enzymes within the boundaries of the yeast cell wall is described and the kinetic properties of hexokinase and pyruvate kinase examined in the permeabilized cells are essentially the same as in cell-free extracts.
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