Gyun Min Lee

TL;DR: The cells appeared to adapt to hyperosmotic stress, and the specific growth rate improved gradually for the first four batches and thereafter, remained constant at 0.040±0.003 (average ± standard deviation) hr−1 which is close to the value obtained from a standard medium in the batch culture.

TL;DR: The effect of serum type and concentration on recombinant protein expression in vaccinia virus infected HeLa S3 cells was studied in both static and suspension culture, indicating that the cultivation method had little influence on β-gal production.

TL;DR: This work revised TSS annotations for 15,308 Chinese hamster genes and 4,478 non-coding RNAs based on experimental data from CHO-K1 cells and 10 hamster tissues and demonstrates, by targeting the glycosyltransferase gene Mgat3, how accurate annotations readily facilitate activating silent genes by CRISPRa to obtain more human-like Glycosylation.

TL;DR: A stable HP recombinant HEK293 cell line can be established using DHFR/MTX‐mediated gene amplification together with dhfr−HEK293 host cells using the limiting dilution method.

TL;DR: Transient gene expression in Chinese hamster ovary (CHO) cells with polyethylenimine (PEI) as a transfection reagent is considered as an attractive method to produce recombinant proteins rapidly for pre-clinical studies, but PEI induces cytotoxicity in a dose-dependent manner.