Showing papers by "Harry F. Noller published in 2006"
TL;DR: In vitro assays show that affinity-purified Deltah69 ribosomes have normal translational accuracy, synthesize a full-length protein from a natural mRNA template, and support EF-G-dependent translocation at wild-type rates, however, Deltahs 50S sub units are unable to associate with 30S subunits in the absence of tRNA, are defective in RF1-catalyzed peptide release, and can be recycled in the absent of RRF
103 citations
TL;DR: Crystallographic studies from the Ramakrishnan laboratory have shown that G530 loop, A1492 and A1493 undergo localized conformational changes to form an RNA structure that positions these three bases to inspect the accuracy of the codon-anticodon match with high stereochemical precision, using A-minor interactions.
35 citations
TL;DR: The structure and dynamics of ribosomal RNA in the Escherichia coli ribosome is probed using equilibrium and time-resolved hydroxyl radical (OH) RNA footprinting to explore changes in the solvent-accessible surface of the rRNA with single-nucleotide resolution to better understand the structural transitions that accompany association of the 30 S and 50 S subunits.
21 citations
09 Feb 2006
TL;DR: In this article, the authors discuss the development of integrated optical sensors with single molecule detection sensitivity based on liquid-core antiresonant reflecting optical waveguides (ARROWs) that allow for simultaneously guiding light and molecules in liquid solution through micron-sized channels on a chip.
Abstract: We discuss the development of novel integrated optical sensors with single molecule detection sensitivity. These sensors are based on liquid-core antiresonant reflecting optical waveguides (ARROWs) that allow for simultaneously guiding light and molecules in liquid solution through micron-sized channels on a chip. Using liquid-core ARROWs as the main building block, two-dimensional planar sensor arrays with sensitivity down to the single molecule level can be fabricated. We present the basic design principle for ARROW waveguides and methods to improve waveguide loss. The influence of surface roughness on the waveguide loss is described. We discuss highly efficient fluorescence detection in both one and two dimensional planar waveguide geometries. Avenues towards subsequent integration with microfluidic systems are presented.
7 citations