H
Hennie R. Hoogenboom
Researcher at Maastricht University
Publications - 85
Citations - 18195
Hennie R. Hoogenboom is an academic researcher from Maastricht University. The author has contributed to research in topics: Phage display & Antibody. The author has an hindex of 51, co-authored 85 publications receiving 17946 citations.
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Journal ArticleDOI
Somatostatin displayed on filamentous phage as a receptor-specific agonist.
Mat Rousch,Jan T. Lutgerink,James E. Coote,Adriaan P. de Bruïne,Jan-Willem Arends,Hennie R. Hoogenboom +5 more
TL;DR: Binding of somatostatin displaying phage to sst2 on a reporter cell line, in which binding of natural ligand reduces secretion of alkaline phosphatase (via a cyclic AMP responsive element sensitive promoter), proved that the phage particles act as receptor‐specific agonists.
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Evidence for a bias toward intracellular antigens in the local humoral anti-tumor immune response of a colorectal cancer patient revealed by phage display.
R C Roovers,Edith van der Linden,Han Zijlema,Adriaan P. de Bruïne,Jan-Willem Arends,Hennie R. Hoogenboom +5 more
TL;DR: Data suggest a bias in the local humoral immune response in this CRC patient, directed primarily toward intracellular epithelial‐cell specific target antigens.
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Oligonucleotide-assisted cleavage and ligation: a novel directional DNA cloning technology to capture cDNAs. Application in the construction of a human immune antibody phage-display library
Sonia Schoonbroodt,Nicolas Frans,Mark DeSouza,Rachel Eren,Smadar Priel,Naama Brosh,Judith Ben-Porath,Arie Zauberman,Ehud Ilan,Shlomo Dagan,Edward H. Cohen,Hennie R. Hoogenboom,Robert Charles Ladner,Rene Hoet +13 more
TL;DR: Full representation of all VH families/segments was observed showing that ONCL did not introduce cloning biases for or against any VH family, and the efficiency of ONCL was validated by creating a functional Fab phage-display library with a size of 3.3 × 1010 and by selecting five unique Fabs against GAPDH antigen.
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Identification of colon tumour-associated antigens by phage antibody selections on primary colorectal carcinoma.
TL;DR: To identify cell-surface targets on colorectal carcinoma (CRC), a large, human phage antibody repertoire was selected on freshly isolated colon tumour cells and two antibodies were identified that reacted with epithelial cell-restricted cell- surface antigens, whereas one clone preferentially reacted with stromal cells.
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Cloning and expression of human V-genes derived from phage display libraries as fully assembled human anti-TNFα monoclonal antibodies
TL;DR: A cloning strategy for the stable expression of scFv or Fab genes isolated from phage display libraries as assembled human antibodies of the IgG1 subclass in Sp2/0 myeloma cells has been described.