Hennie R. Hoogenboom

TL;DR: Five human single-chain Fv antibodies specific for the MUC1 variable number of tandem repeats region are isolated by in vitro selection from a large naive phage antibody library containing over 6 x 10(9) different scFv antibodies.

TL;DR: Interestingly, only a single hydrogen bond between a peptide side chain and Fab-Hyb3 contributes to the interaction, but large buried surface areas with pronounced shape complementarity assure high affinity and specificity for MAGE-A1.

TL;DR: The rat membrane protein fatty acid translocase (FAT), which shows sequence similarity to human CD36 (a membrane protein supposedly involved in a variety of membrane processes), is implicated in the transport of long-chain fatty acids across cellular membranes and phage display technology has been succesfully applied for direct selection of antibodies that recognize selected membrane proteins in their natural context.

TL;DR: Two model selection systems based on phage antibodies directed to the tumour antigen epithelial glycoprotein-2 and a model based on a phage antibody directed against the endothelial cell inducible adhesion molecule E-selectin are described.

TL;DR: This small and partially humanized recombinant bispecific antibody fragment may be valuable for T‐cell–based immunotherapeutical treatment protocols, retargeting activated peripheral blood T lymphocytes to lyse various human carcinomas in vivo.