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William F. Bennett
Researcher at Genentech
Publications - 42
Citations - 2245
William F. Bennett is an academic researcher from Genentech. The author has contributed to research in topics: Tissue plasminogen activator & Plasminogen activator. The author has an hindex of 15, co-authored 42 publications receiving 2224 citations.
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Journal ArticleDOI
Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli
Diane Pennica,William E. Holmes,William J. Kohr,Richard N. Harkins,Gordon A. Vehar,Carole A. Ward,William F. Bennett,Elizabeth Mcleod Yelverton,Peter H. Seeburg,Herbert L. Heyneker,David V. Goeddel,Desire Collen +11 more
TL;DR: Bacterial clones containing human tissue-type plasminogen activator cDNA sequences were identified in a cDNA library prepared using gel-fractionated mRNA from human melanoma cells and a polypeptide was produced having the fibrinolytic properties characteristic of authentic human t-PA.
Journal ArticleDOI
High resolution analysis of functional determinants on human tissue-type plasminogen activator
William F. Bennett,Nicholas F. Paoni,Bruce Keyt,David Botstein,A. J. S. Jones,L. Presta,Florian M. Wurm,Mark J. Zoller +7 more
TL;DR: Variants of tPA were found that had reduced activity with respect to each tested property; in a few cases increased activity was observed, and a model of the tPA protease domain has been used to map some of the critical residues and regions.
Journal Article
Biological properties of human tissue-type plasminogen activator obtained by expression of recombinant DNA in mammalian cells.
D. Collen,Jm Stassen,B J Marafino,S Builder,F De Cock,J Ogez,D Tajiri,Diane Pennica,William F. Bennett,J. Salwa +9 more
TL;DR: It is concluded that the potentially more readily available rt-PA could constitute a specific, fibrin-selective thrombolytic agent.
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A long-half-life and fibrin-specific form of tissue plasminogen activator in rabbit models of embolic stroke and peripheral bleeding.
G R Thomas,Harold Thibodeaux,C J Errett,J M Badillo,Bruce Keyt,Canio J. Refino,J A Zivin,William F. Bennett +7 more
TL;DR: Findings show that by combining increased fibrin specificity with decreased plasma clearance, it is possible to produce a thrombolytic agent that is more convenient and more potent than wild-type TPA.
Journal ArticleDOI
Identification of carbohydrate structures in glycoprotein peptide maps by the use of LC/MS with selected ion extraction with special reference to tissue plasminogen activator and a glycosylation variant produced by site directed mutagenesis
TL;DR: Electrospray ionization mass spectrometry utilizing a single quadrupole on line with reversed-phase HPLC (LC/MS) enables the characterization of glycoproteins in a relatively short period of time.