T
Tsutomu Arie
Researcher at Tokyo University of Agriculture and Technology
Publications - 138
Citations - 3819
Tsutomu Arie is an academic researcher from Tokyo University of Agriculture and Technology. The author has contributed to research in topics: Fusarium oxysporum & Gene. The author has an hindex of 28, co-authored 129 publications receiving 3192 citations. Previous affiliations of Tsutomu Arie include University of Tokyo.
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Journal ArticleDOI
Molecular organization of mating type loci in heterothallic, homothallic, and asexual Gibberella/Fusarium species.
TL;DR: RT-PCR analysis proved that the F. oxysporum MAT genes are expressed and that all putative introns found in each of the four MAT genes in G. fujikuroi could not be attributed to mutations in the MAT genes themselves.
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Large-scale analysis of full-length cDNAs from the tomato ( Solanum lycopersicum ) cultivar Micro-Tom, a reference system for the Solanaceae genomics
Koh Aoki,Kentaro Yano,Ayako Suzuki,Shingo Kawamura,Nozomu Sakurai,Kunihiro Suda,Atsushi Kurabayashi,Tatsuya Suzuki,Taneaki Tsugane,Manabu Watanabe,Kazuhide Ooga,Maiko Torii,Takanori Narita,Tadasu Shin-I,Yuji Kohara,Naoki Yamamoto,Hideki Takahashi,Yuichiro Watanabe,Mayumi Egusa,Motoichiro Kodama,Yuki Ichinose,Mari Kikuchi,Sumire Fukushima,Akiko Okabe,Tsutomu Arie,Yuko Sato,Katsumi Yazawa,Shinobu Satoh,Toshikazu Omura,Hiroshi Ezura,Daisuke Shibata +30 more
TL;DR: The collection of Micro-Tom nrFLcDNAs generated in this study will serve as a valuable genomic tool for plant biologists to bridge the gap between basic and applied studies and aid in tomato functional genomics and molecular breeding.
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Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus
Takayuki Arazoe,Kennosuke Miyoshi,Tohru Yamato,Tetsuo Ogawa,Shuichi Ohsato,Tsutomu Arie,Shigeru Kuwata +6 more
TL;DR: It is demonstrated that RGNs could recognize the desired sequences and edit endogenous genes through homologous recombination‐mediated targeted gene replacement with high efficiency and will open the way for the development of various CRISPR/Cas‐based applications in filamentous fungi.
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Efficient cloning of Ascomycete mating type genes by PCR amplification of the conserved MAT HMG box
TL;DR: Cloning of mating type (MAT) genes from ascomycetes has been hampered by low conservation among them, but regions of the MAT locus flanking the HMG box were readily cloned using the TAIL-PCR procedure with a combination of random and specific primers.
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Mating-type genes from asexual phytopathogenic ascomycetes Fusarium oxysporum and Alternaria alternata
TL;DR: Mating-type (MAT) loci were cloned from two asexual (mitosporic) phytopathogenic ascomycetes, Fusarium oxysporum and Alternaria alternata, by a polymerase chain reaction (PCR)-based strategy and were confirmed to be functional in a close sexual relative, Cochliobolus heterostrophus, by heterologous expression.