Hisasi Kikuchi

TL;DR: The complete sequence of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3, has been determined by assembling the sequences of the physical map-based contigs of fosmid clones and of long polymerase chain reaction (PCR) products which were used for gap-filling.

TL;DR: The complete sequence of the genome of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1, which optimally grows at 95 degrees C, has been determined by the whole genome shotgun method with some modifications.

TL;DR: Sequence comparison provided evidence suggesting the integration of plasmid, rearrangement of genomic structure, and duplication of genomic regions that may be responsible for the larger genomic size of the S. tokodaii strain7 genome.

TL;DR: Genes encoding both an archaeal and a bacterial class I enzyme were able to rescue an Escherichia coli strain deficient in LysRS, indicating their ability to functionally substitute for a class II LysRS in vivo.

TL;DR: The crystal structure of this aminotransferase from the hyperthermophilic archaeon Pyrococcus horikoshii was determined and it was found that the enzyme recognizes the aromatic substrate by hydrophobic interaction with aromatic rings (Phe121 and Tyr59*) and probably recognizes acidic substrates by a hydrophilic interaction involving a hydrogen bond network with Thr264*.