Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1.
Yutaka Kawarabayasi,Yumi Hino,Hirosh Horikawa,Syuji Yamazaki,Yuji Haikawa,Koji Jin-no,Mikio Takahashi,Mitsuo Sekine,Sin-ichi Baba,Akiho Ankai,Hiroki Kosugi,Akira Hosoyama,Shigehiro Fukui,Yoshimi Nagai,Keiko Nishijima,Hidekazu Nakazawa,Minako Takamiya,Sayaka Masuda,Tomomichi Funahashi,Toshihiro Tanaka,Yutaka Kudoh,Jun Yamazaki,Norihiro Kushida,Akio Oguchi,Ken-ichi Aoki,Kenji Kubota,Yoshinobu Nakamura,Norimichi Nomura,Yoshihiko Sako,Hisasi Kikuchi +29 more
TLDR
The complete sequence of the genome of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1, which optimally grows at 95 degrees C, has been determined by the whole genome shotgun method with some modifications.Abstract:
The complete sequence of the genome of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix Kl, which optimally grows at 95°C, has been determined by the whole genome shotgun method with some modifications. The entire length of the genome was 1,669,695 bp. The authenticity of the entire sequence was supported by restriction analysis of long PCR products, which were directly amplified from the genomic DNA. As the potential protein-coding regions, a total of 2,694 open reading frames (ORFs) were assigned. By similarity search against public databases, 633 (23.5%) of the ORFs were related to genes with putative function and 523 (19.4%) to the sequences registered but with unknown function. All the genes in the TCA cycle except for that of alpha-ketoglutarate dehydrogenase were included, and instead of the alpha-ketoglutarate dehydrogenase gene, the genes coding for the two subunits of 2-oxoacid:ferredoxin oxidoreductase were identified. The remaining 1,538 ORFs (57.1%) did not show any significant similarity to the sequences in the databases. Sequence comparison among the assigned ORFs suggested that a considerable member of ORFs were generated by sequence duplication. The RNA genes identified were a single 16S-23S rRNA operon, two 5S rRNA genes and 47 tRNA genes including 14 genes with intron structures. All the assigned ORFs and RNA coding regions occupied 89.12% of the whole genome. The data presented in this paper are available on the internet homepage (http://www.mild.nite.go.jp).read more
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