Hong Zhang

TL;DR: The high consistence between the proposed approach RCA-assisted CRISPR/Cas9 cleavage (RACE) and reverse transcription quantitative polymerase chain reaction (RT-qPCR) in detecting EV-derived miRNAs abundance from both cultured cancer cells and clinical lung cancer patients validated its robustness, revealing its potentials in the screening, diagnosis and prognosis of various diseases.

TL;DR: This review of GQDs-based nanohybrid materials focuses on the most recent emerging developments including synthesis methods, in vivo imaging and in vitro biosensing applications, and unresolved problematic and controversial issues facing their biomedical applications.

TL;DR: An overview of the current knowledge of DSNs is presented, with special emphasis on DSN-mediated isothermal signal amplification strategies for trace miRNA detection and improved personalized medicine, preventive medicine, and translational medicine.

TL;DR: An amplification-free strategy for real-time monitoring of miRNA-DNA hybridization that integrates the advantages of both duplex-specific nuclease (DSN)-triggered signal amplification and single-stranded DNA probe coating facilitated by reduced graphene oxide is developed.

TL;DR: In this review, several methods including ultracentrifugation and precipitation for isolating OMVs were summarized and the importance of genetic engineering for the safe and effective application and in facilitating the rapid development of OMVs was highlighted.