Huamei Zhang

TL;DR: The systematic identification of protein interactions for the bacterium Campylobacter jejuni is reported, one of the most comprehensive yet determined for a free-living organism and nearly doubles the binary interactions available for the prokaryotic kingdom.

TL;DR: The substantial number of new interactions discovered here supports the conclusion that previous interaction mapping studies were far from complete and that many more interactions remain to be found.

TL;DR: High-throughput yeast two-hybrid screening is used to identify mosquito and human proteins that physically interact with dengue proteins and identified a subset of the host-dengue interactions that are now supported by multiple forms of evidence, which should be useful for understanding the interplay between d Dengue and its hosts.

TL;DR: An efficient two-hybrid strategy to facilitate construction of protein interaction maps on a genome-wide scale that has several advantages over previously described approaches, including the ability to detect interactions with proteins that inhibit yeast growth or that activate transcription as BD fusions.

TL;DR: A conditional expression system that takes advantage of the yeast MAL62 promoter (MAL62p), which can be controlled by adding maltose or glucose to the growth medium to induce or repress transcription, and a mating scheme is used to dramatically increase the dynamic range of expression levels possible.