H
Hugh E. Olsen
Researcher at University of California, Santa Cruz
Publications - 51
Citations - 7097
Hugh E. Olsen is an academic researcher from University of California, Santa Cruz. The author has contributed to research in topics: Nanopore sequencing & Minion. The author has an hindex of 23, co-authored 45 publications receiving 5389 citations. Previous affiliations of Hugh E. Olsen include University of California, Berkeley & University of California, Davis.
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Journal ArticleDOI
Nanopore sequencing and assembly of a human genome with ultra-long reads
Miten Jain,Sergey Koren,Karen H. Miga,Josh Quick,Arthur C Rand,Thomas A Sasani,John R. Tyson,Andrew D Beggs,Alexander T. Dilthey,Ian T. Fiddes,Sunir Malla,Hannah Marriott,Tom Nieto,Justin O'Grady,Hugh E. Olsen,Brent S. Pedersen,Arang Rhie,Hollian Richardson,Aaron R. Quinlan,Terrance P. Snutch,Louise Tee,Benedict Paten,Adam M. Phillippy,Jared T. Simpson,Jared T. Simpson,Nicholas J. Loman,Matthew Loose +26 more
TL;DR: Ultra-long reads enabled assembly and phasing of the 4-Mb major histocompatibility complex (MHC) locus in its entirety, measurement of telomere repeat length, and closure of gaps in the reference human genome assembly GRCh38.
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The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community
TL;DR: Key technical features of the Oxford Nanopore MinION, the dominant platform currently available, are summarized and pioneering applications executed by the genomics community are discussed.
Journal ArticleDOI
Improved data analysis for the MinION nanopore sequencer
TL;DR: This work presents a single-nucleotide-variant detection tool that uses maximum-likelihood parameter estimates and marginalization over many possible read alignments to achieve precision and recall of up to 99% by pairing the authors' high-confidence alignment strategy with long MinION reads.
Journal ArticleDOI
Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channel.
Wenonah Vercoutere,Stephen Winters-Hilt,Hugh E. Olsen,David W. Deamer,David Haussler,Mark Akeson +5 more
TL;DR: This nanopore detector with a support vector machine (SVM) to analyze DNA hairpin molecules on the millisecond time scale and can discriminate between individual DNA hairpins that differ by one base pair or by one nucleotide.
Journal ArticleDOI
Mapping DNA methylation with high-throughput nanopore sequencing
Arthur C Rand,Miten Jain,Jordan M. Eizenga,Audrey Musselman-Brown,Hugh E. Olsen,Mark Akeson,Benedict Paten +6 more
TL;DR: The results show that the model is sensitive enough to detect changes in genomic DNA methylation levels as a function of growth phase in Escherichia coli.