Huili Guo

TL;DR: Results show that changes in mRNA levels closely reflect the impact of miRNAs on gene expression and indicate that destabilization of target mRNAs is the predominant reason for reduced protein output.

TL;DR: In this paper, the authors used ribosome profiling to measure the overall effects on protein production and compare these to simultaneously measured effects on mRNA levels, showing that changes in mRNA levels closely reflect the impact of miRNAs on gene expression and indicate that destabilization of target mRNAs is the predominant reason for reduced protein output.

TL;DR: A computational pipeline to identifycircRNAs and quantify their relative abundance from RNA-seq data is developed, providing a new framework for future investigation of this intriguing topological isoform while raising doubts regarding a biological function of most circRNAs.

TL;DR: The global steady-state measurements are extended to additional mammalian contexts and find that regardless of the miRNA, cell type, growth condition, or translational state, mRNA destabilization explains most (66%->90%) miRNA-mediated repression.

TL;DR: It is reported that siRNAs derived from long hairpin RNA genes (hpRNAs) programme Slicer complexes that can repress endogenous target transcripts and reveal unexpected complexity in the sorting of small RNAs in Drosophila.