N
Nicholas T. Ingolia
Researcher at University of California, Berkeley
Publications - 114
Citations - 25377
Nicholas T. Ingolia is an academic researcher from University of California, Berkeley. The author has contributed to research in topics: Translation (biology) & Ribosome profiling. The author has an hindex of 51, co-authored 103 publications receiving 22011 citations. Previous affiliations of Nicholas T. Ingolia include Howard Hughes Medical Institute & Carnegie Institution for Science.
Papers
More filters
Mammalian microRNAs predominantly act to decrease target mRNA levels
Huili Guo,Nicholas T. Ingolia,Nicholas T. Ingolia,Jonathan S. Weissman,Jonathan S. Weissman,David P. Bartel +5 more
TL;DR: Results show that changes in mRNA levels closely reflect the impact of miRNAs on gene expression and indicate that destabilization of target mRNAs is the predominant reason for reduced protein output.
Journal ArticleDOI
Genome-Wide Analysis in Vivo of Translation with Nucleotide Resolution Using Ribosome Profiling
TL;DR: A ribosomesome-profiling strategy based on the deep sequencing of ribosome-protected mRNA fragments is presented and enables genome-wide investigation of translation with subcodon resolution and is used to monitor translation in budding yeast under both rich and starvation conditions.
Journal ArticleDOI
Mammalian microRNAs predominantly act to decrease target mRNA levels
Huili Guo,Nicholas T. Ingolia,Nicholas T. Ingolia,Jonathan S. Weissman,Jonathan S. Weissman,David P. Bartel +5 more
TL;DR: In this paper, the authors used ribosome profiling to measure the overall effects on protein production and compare these to simultaneously measured effects on mRNA levels, showing that changes in mRNA levels closely reflect the impact of miRNAs on gene expression and indicate that destabilization of target mRNAs is the predominant reason for reduced protein output.
Journal ArticleDOI
Ribosome Profiling of Mouse Embryonic Stem Cells Reveals the Complexity and Dynamics of Mammalian Proteomes
TL;DR: A suite of techniques, based on ribosome profiling, are presented to provide genome-wide maps of protein synthesis as well as a pulse-chase strategy for determining rates of translation elongation, revealing an unanticipated complexity to mammalian proteomes.
Journal ArticleDOI
The translational landscape of mTOR signalling steers cancer initiation and metastasis
Andrew C. Hsieh,Yi Liu,Merritt Edlind,Nicholas T. Ingolia,Matthew R. Janes,Annie Sher,Evan Y. Shi,Craig R. Stumpf,Carly Christensen,Michael Bonham,Shunyou Wang,Pingda Ren,Michael C. Martin,Katti Jessen,Morris E. Feldman,Jonathan S. Weissman,Kevan M. Shokat,Christian Rommel,Davide Ruggero +18 more
TL;DR: A clinically relevant ATP site inhibitor of mTOR, INK128, is developed, which reprograms this gene expression signature with therapeutic benefit for prostate cancer metastasis, for which there is presently no cure.