Vikram Agarwal

TL;DR: It is shown that recently reported non-canonical sites do not mediate repression despite binding the miRNA, which indicates that the vast majority of functional sites are canonical.

TL;DR: A computational pipeline to identifycircRNAs and quantify their relative abundance from RNA-seq data is developed, providing a new framework for future investigation of this intriguing topological isoform while raising doubts regarding a biological function of most circRNAs.

TL;DR: Modulation of miRNA target abundance is unlikely to cause significant effects on gene expression and metabolism through a ceRNA effect.

TL;DR: Although miRNA targeting is remarkably consistent in different cell types, considering the 3' UTR landscape helps predict targeting efficacy and explain differential regulation that is observed.

TL;DR: Although miRNA levels define the extent of repression, they have little effect on the magnitude of the ceRNA expression change required to observe derepression, and quantitative insights are provided into the stoichiometric relationship between miRNAs and target abundance, target-site spacing, and affinity requirements for ceRNA-mediated gene regulation.