Ido Golding

TL;DR: This work directly demonstrates transcriptional bursting in Escherichia coli, similar to that indirectly inferred for eukaryotes, and extends protein-based approaches by counting the integer-valued number of transcript with single-molecule resolution.

TL;DR: This work tracks the motion of individual fluorescently labeled mRNA molecules inside live E. coli cells and finds that the motion is subdiffusive, with an exponent that is robust to physiological changes, including the disruption of cytoskeletal elements.

TL;DR: Evidence from genome-wide noise studies and from systematic perturbations of promoter sequences suggest that both scenarios—namely gene-specific versus genome- wide regulation of transcription kinetics—may be present to different degrees in bacteria, yeast, and animal cells.

TL;DR: It is demonstrated that a newly developed optical interferometric technique, known as spatial light interference microscopy, can measure the cell dry mass of many individual adherent cells in various conditions, over spatial scales from micrometers to millimeters, temporal scales ranging from seconds to days, and cell types ranging from bacteria to mammalian cells.

TL;DR: This work quantifies copy-number statistics of mRNA from 20 Escherichia coli promoters using single-molecule fluorescence in situ hybridization to characterize the general properties of transcriptional time series and finds that the degree of burstiness is correlated with gene expression level but is largely independent of other parameters of gene regulation.